Role of in vitro factors in ozone toxicity for cultured rat lung fibroblasts.
Role of in vitro factors in ozone toxicity for cultured rat lung fibroblasts.
复制标题
体外因素在臭氧对培养大鼠肺成纤维细胞毒性中的作用。
DOI：
10.3109/01480548209041053
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发表时间：
1982
影响因子：
2.6
通讯作者：
D. Morgan
中科院分区：
文献类型：
--
作者：
D. G. Wenzel;D. Morgan
臭氧对培养的大鼠肺成纤维细胞的毒性是浓度依赖的，并受到臭氧输送到细胞的方式的影响，即培养物要么旋转，覆盖一层薄薄的移动介质，要么在细胞和气相之间固定一层固定的介质。研究了培养基组分和培养皿组成对臭氧毒性的影响。用来衡量臭氧毒性的细胞活力是通过铬-51释放试验和活力指数来量化的，该指数是根据被活性染料染色的细胞的百分比结合DNA测量确定的细胞数量减少来计算的。在固定臭氧暴露期间，毒性似乎主要是由过氧化氢介导的，当以生存指数衡量时，过氧化氢酶或胎牛血清可以抑制毒性。在轮换暴露期间，过氧化氢酶和胎牛血清在以生存指数衡量时不起保护作用，而当用铬-51释放法测定时，胎牛血清有部分保护作用。臭氧对成纤维细胞的影响不受培养皿是玻璃还是塑料的影响，也不受培养介质是平衡盐溶液还是完全化学定义的介质的影响。
Ozone toxicity for cultured rat lung fibroblasts was concentration dependent and was affected by the manner in which ozone was delivered to the cells, i.e. cultures were either rotated with a thin moving overlay of medium or were stationary with a fixed layer of medium between the cells and the gas phase. The influence of culture medium components and culture dish composition on the toxicity of ozone were also investigated. Cell viability, used to measure ozone toxicity, was quantified by the chromium-51 release assay, and by a viability index calculated from the percentage of cells stained with a vital dye combined with the decrease in cell number as determined by DNA measurements. During stationary ozone exposure, toxicity appeared to be mediated primarily by hydrogen peroxide and could be inhibited by catalase or fetal bovine serum when measured by the viability index. During rotated exposure, catalase and fetal bovine serum provided no protection when measured by the viability index, however, when measured by the chromium-51 release assay, fetal bovine serum was partially protective. The effect of ozone on the fibroblasts was not influenced by whether culture dishes were glass or plastic, or whether the culture medium was balanced salt solution or complete chemically-defined medium.
过氧化氢会对暴露于氧自由基的人体成纤维细胞造成致命伤害。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Simon,RH;Scoggin,CH;Patterson,D
通讯作者：
Patterson,D