Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii.
Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii.
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DOI：
10.1093/genetics/118.4.637
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发表时间：
1988-04
期刊：
影响因子：
3.3
通讯作者：
S. Barsel;D. Wexler;P. Lefebvre
中科院分区：
文献类型：
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作者：
S. Barsel;D. Wexler;P. Lefebvre
莱茵衣藻细胞的鞭毛长度受到严格的调控，短鞭毛和长鞭毛突变体都有描述。本报告描述了10个长鞭毛突变体，包括5个新分离的突变体，以确定赋予这种表型的不同位点的数量，并研究在不同位点的突变体的相互作用。这些突变体在杂合二倍体和野生型中均为隐性，可分为三个不连锁互补群。其中之一定义了一个新的基因，lf 3，它定位在连锁群I的着丝粒附近。每个突变体的群体中的鞭毛长度分布是广泛的，最长的鞭毛测量四倍的长度上看到的野生型细胞的最长鞭毛。10个突变体中的每一个在截肢后都有鞭毛再生缺陷。一些突变体在野生型细胞完全再生鞭毛所需的时间内没有再生长。其他突变体具有快速再生动力学的亚群，以及没有观察到再生的亚群。每个突变体杂交野生型形成临时的四鞭毛，双核细胞;在每种情况下，长鞭毛迅速缩短野生型细胞质的存在下，表明突变体是隐性的，和长度控制可以施加在已经组装的鞭毛。
The length of the flagella of Chlamydomonas reinhardtii cells is tightly regulated; both short-flagella and long-flagella mutants have been described. This report characterizes ten long-flagella mutants, including five newly isolated mutants, to determine the number of different loci conferring this phenotype, and to study interactions of mutants at different loci. The mutants, each of which was recessive in heterozygous diploids with wild type, fall into three unlinked complementation groups. One of these defines a new gene, lf3, which maps near the centromere of linkage group I. The flagellar length distributions in populations of each mutant were broad, with the longest flagella measuring four times the length of the longest flagella seen on wild-type cells. Each of the ten mutants had defective flagellar regrowth after amputation. Some of the mutants showed no regrowth within the time required for wild-type cells to regenerate flagella completely. Other mutants had subpopulations with rapid regeneration kinetics, and subpopulations with no observable regeneration. The mutants were each crossed to wild type to form temporary quadriflagellate, dikaryon cells; in each case the long flagella were rapidly shortened in the presence of the wild-type cytoplasm, demonstrating that the mutants were recessive, and that length control could be exerted on already assembled flagella.