Cloning, sequence analysis and tissue distribution of the mouse and rat urotensin II precursors
Cloning, sequence analysis and tissue distribution of the mouse and rat urotensin II precursors
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DOI：
10.1016/s0014-5793(99)01003-0
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发表时间：
1999-08-20
期刊：
影响因子：
3.5
通讯作者：
Lihrmann, I
中科院分区：
文献类型：
--
作者：
Coulouarn, Y;Jégou, S;Lihrmann, I
尾加压素 II (UII) 是一种环状神经肽，最初从硬骨鱼的尾部神经分泌系统中分离出来。最近在青蛙和人类中克隆了 UII 前体，证明该肽不仅限于鱼类的尿生肌，而且也在四足动物的中枢神经系统中表达。在这里，我描述了小鼠和大鼠中编码 prepro-UII 的 cDNA 的特征。将小鼠和大鼠UII的一级结构与其他脊椎动物UII的一级结构进行比较表明，该分子的环状区域（CFWKYC）的序列完全保守。相反，prepro-UII的N端侧翼结构域存在显着差异，小鼠或大鼠与人类前体之间只有48％的序列同一性。原位杂交组织化学显示prepro-UII基因主要在脑干和脊髓的运动神经元中表达，表明UII可能在神经肌肉功能的控制中发挥作用。 (C) 1999 年欧洲生化学会联合会。
Urotensin II (UII) is a cyclic neuropeptide initially isolated from the caudal neurosecretory system of teleost fish., The recent cloning of the UII precursor in frog and human has demonstrated that the peptide is not restricted to the fish urophysis but that it is also expressed in the central nervous system of tetrapods. Here, me describe the characterization of the cDNAs encoding prepro-UII in mouse and rat. A comparison of the primary structures of mouse and rat UII with those of other vertebrate UII reveals that the sequence of the cyclic region of the molecule (CFWKYC) has been fully conserved, In contrast, the N-terminal flanking domain of prepro-UII has markedly diverged with only 48% sequence identity between the mouse or rat and the human precursors. In situ hybridization histochemistry showed that the prepro-UII gene is predominantly expressed in motoneurons of the brainstem and spinal cord, suggesting that UII may play a role in the control of neuromuscular functions. (C) 1999 Federation of European Biochemical Societies.