Regulatory roles of biogenic amines and juvenile hormone in the reproductive behavior of the western tarnished plant bug (Lygus hesperus)
Regulatory roles of biogenic amines and juvenile hormone in the reproductive behavior of the western tarnished plant bug (Lygus hesperus)
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DOI：
10.1007/s00360-015-0953-1
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发表时间：
2016-02-01
影响因子：
2
通讯作者：
Nath, Rachna
中科院分区：
文献类型：
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作者：
Brent, Colin S.;Miyasaki, Katelyn;Nath, Rachna
交配引起了植物小蝽（半翅目：盲蝽科）的行为和生理变化。在接受了包括系统性调节激素（JH）在内的精液产品后，雌性进入了持续数天的交配后期，在此期间，它们的产卵率提高，并失去了交配的兴趣。为了阐明这些行为变化的调控机制，我们在交配后5 min、1 h和24 h对雌性猕猴桃头部的生物胺进行了定量分析，并利用高效液相色谱联用电化学检测方法与未交配雌性猕猴桃头部的生物胺含量进行了比较。交配1 h和24 h后多巴胺（DA）显著升高，5 min和1 h后八胺（OA）显著降低。5 min后血清素不随交配变化，但酪胺显著降低。注射胺对未交配雌虫的性接受性没有影响，但注射后24小时内OA导致产卵减少。将JH类似物甲氧二烯局部应用于雏鸟，会导致DA的增加和交配倾向的下降，但对其他胺或产卵率没有影响。结果表明，交配后立即观察到的OA下降可能会促进产卵，而雄性衍生的JH可能会导致DA增加，这可能解释了交配后性接受性丧失的原因。
Mating induces behavioral and physiological changes in the plant bug Lygus hesperus Knight (Hemiptera: Miridae). After receiving seminal products, which include the systemic regulator juvenile hormone (JH), females enter a post-mating period lasting several days during which they enhance their oviposition rate and lose interest in remating. To elucidate the regulation of these behavioral changes in L. hesperus, biogenic amines were quantified in the heads of females at 5 min, 1 h and 24 h after copulation and compared to levels in virgins using high-performance liquid chromatography coupled with electrochemical detection. Mating significantly increased dopamine (DA) after 1 and 24 h, and decreased octopamine (OA) after 5 min and 1 h. Serotonin did not change with mating, but tyramine was significantly reduced after 5 min. While injection of amines into virgin females did not influence sexual receptivity, OA caused a decrease in oviposition during the 24 h following injection. Topical application of the JH analog methoprene to virgins caused an increase in DA, and a decline in mating propensity, but did not influence other amines or the oviposition rate. The results suggest the decline in OA observed immediately after mating may promote egg laying, and that male-derived JH may induce an increase in DA that could account for the post-mating loss of sexual receptivity.