Occurrence of reticuloendotheliosis in Chinese partridge

Occurrence of reticuloendotheliosis in Chinese partridge
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DOI:
10.1292/jvms.69.1295
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发表时间:
2007-12-01
影响因子:
1.2
通讯作者:
Cui, Zhizhong
Cui, Zhizhong
中科院分区:
农林科学4区
文献类型:
--
作者:
Cheng, Ziqiang;Shi, Youfei;Cui, Zhizhong

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2006 年 6 月,两群商业中华鹧鸪在 120 日龄时出现了与消耗性疾病相关的死亡率增加。死鸡的尸检一致显示内脏组织(主要是腺胃、肝脏和脾脏)弥漫性肿大。镜检发现腺胃、肝、脾、肾、心、肺、胸腺、肠等组织中可见大量未成熟淋巴细胞,核分裂象频繁。从四个血液样本中分别分离出网状内皮增生病毒(REV)。采用间接免疫荧光法在培养的CEF(SPF胚胎,济南家禽研究所)接种的血样中观察到病毒抗原。通过PCR从六只鸡的肝脏样本中获得了REV的LTR的300 bp片段。此前在鹧鸪中未见此病的报道。鹧鸪可能是其他中国本土鸡的潜在感染宿主。
Two commercial flocks of Chinese partridge experienced increased mortality associated with a wasting disease at 120-day old in June 2006. Postmortem examination of dead chickens consistently showed visceral tissues mainly proventriculus, liver and spleen were diffuse enlargement. Microscopic examination revealed masses of immature lymphocytes with frequent mitotic figures were seen in various tissues including proventriculus, liver, spleen, kidney, heart, lung, thymus and intestine. Reticuloendotheliosis virus (REV) was isolated from each of four blood samples. Viral antigens were observed in cultured CEF (SPF embryos came from the Ji-nan poultry institute) inoculated blood samples via on indirect immunofluorescent assay. Three hundred bp fragments of LTR of REV obtained from liver samples of six chickens by PCR. This disease has not previously been reported in Chinese partridge. Chinese partridge may represent a potential reservoir of infection for other Chinese local chickens.