Characterization Based on the 56-Kda Type-Specific Antigen Gene of Orientia tsutsugamushi Genotypes Isolated from Leptotrombidium Mites and the Rodent Host Post-Infection
Characterization Based on the 56-Kda Type-Specific Antigen Gene of Orientia tsutsugamushi Genotypes Isolated from Leptotrombidium Mites and the Rodent Host Post-Infection
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DOI：
10.4269/ajtmh.13-0393
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发表时间：
2014-01-01
影响因子：
3.3
通讯作者：
Richardson, Jason H.
中科院分区：
文献类型：
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作者：
Takhampunya, Ratree;Tippayachai, Bousaraporn;Richardson, Jason H.
对来自三个自然感染的实验室饲养的东方体(Ot)的56 kDa类型特异性抗原(TSA)基因进行了分析，发现存在单一和共存的Ot。在个体吸虫中发现的基因类型。在所有研究对象中均发现Karp基因，而Gilliam和UT302基因仅与Karp基因一起被观察到。通过对这些OT型的分析，确定了LC和LI感染后，啮齿动物脾中的OT型基因组成没有发生改变，因此与饲养型钩体的OT型相似。然而，在携带Karp和Gilliam基因的LD恙鼠喂养后，仅在啮齿动物中发现了Karp基因。目前的发现揭示了宿主、病原体和媒介之间的复杂关联。
Characterization of the 56-kDa type-specific antigen (TSA) genes of Orientia tsutsugamushi (OT) from three naturally infected, laboratory-reared mite colonies comprising three species (Leptotrombidium deliense [Ld], Leptotrombidium imphalum [Li], and Leptotrombidium chiangraiensis [Lc]) has revealed the presence of single and coexisting OT. genotypes found in individual chiggers. The Karp genotype was found in all of the chiggers examined, whereas Gilliam and UT302 genotypes were only observed in combination with the Karp genotype. From analysis of these OT genotypes after transmission from chiggers to mice it was determined that with the Lc and Li mites, the OT genotype composition in the rodent spleens post-infection had not changed and therefore resembled that observed in the feeding chiggers. However, only the Karp genotype was found in rodents after feeding by Ld chiggers carrying Karp and Gilliam genotypes. The current findings reveal a complex association among the host, pathogen, and vector.