Complete DNA barcode reference library for a country's butterfly fauna reveals high performance for temperate Europe
Complete DNA barcode reference library for a country's butterfly fauna reveals high performance for temperate Europe
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DOI：
10.1098/rspb.2010.1089
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发表时间：
2011-02-07
影响因子：
4.7
通讯作者：
Vila, Roger
中科院分区：
文献类型：
--
作者：
Dinca, Vlad;Zakharov, Evgeny V.;Vila, Roger
DNA条形码旨在加速物种识别和发现，但性能测试显示识别成功率存在显著差异。因此，仍然非常需要进行全面的研究，客观地测试具有坚实分类框架的群体方法。这项研究的重点是罗马尼亚的180种蝴蝶，约占欧洲蝴蝶区系的三分之一。这个国家包括五个生态区，是欧盟最高的，是温带地区的良好代表。对1300多份标本的形态和DNA条形码进行了仔细研究和比较。我们的研究结果表明，90%的物种形成条形码集群，使其可靠的识别。其余的案例涉及9个密切相关的物种对，其中一些物种的分类地位是有争议的或经常杂交。有趣的是，DNA条形码被发现是最有效的识别工具，优于外部形态学，并略优于男性生殖器。罗马尼亚现在是第一个拥有全面的蝴蝶DNA条形码参考数据库的国家。在对特定地理区域进行全面抽样的基础上进行类似的条形码工作，可以作为功能模块，在全球系统正在开发的同时，促进DNA条形码的早期应用。
DNA barcoding aims to accelerate species identification and discovery, but performance tests have shown marked differences in identification success. As a consequence, there remains a great need for comprehensive studies which objectively test the method in groups with a solid taxonomic framework. This study focuses on the 180 species of butterflies in Romania, accounting for about one third of the European butterfly fauna. This country includes five eco-regions, the highest of any in the European Union, and is a good representative for temperate areas. Morphology and DNA barcodes of more than 1300 specimens were carefully studied and compared. Our results indicate that 90 per cent of the species form barcode clusters allowing their reliable identification. The remaining cases involve nine closely related species pairs, some whose taxonomic status is controversial or that hybridize regularly. Interestingly, DNA barcoding was found to be the most effective identification tool, outperforming external morphology, and being slightly better than male genitalia. Romania is now the first country to have a comprehensive DNA barcode reference database for butterflies. Similar barcoding efforts based on comprehensive sampling of specific geographical regions can act as functional modules that will foster the early application of DNA barcoding while a global system is under development.