Application of a C-peptide electrospray ionization-isotope dilution-liquid chromatography-tandem mass spectrometry measurement procedure for the evaluation of five C-peptide immunoassays for urine
Application of a C-peptide electrospray ionization-isotope dilution-liquid chromatography-tandem mass spectrometry measurement procedure for the evaluation of five C-peptide immunoassays for urine
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DOI：
10.1016/s1570-0232(03)00268-x
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发表时间：
2003-07-25
影响因子：
3
通讯作者：
Thienpont, LM
中科院分区：
文献类型：
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作者：
Fierens, C;Stöckl, D;Thienpont, LM
本研究应用电喷雾电离-同位素稀释-液相色谱-串联质谱法通过分割样品测量来评估五种尿液 C 肽免疫测定。免疫测定在同一运行中重复测量，比较方法在不同运行中重复三次。根据数据计算批内不精密度和方法比较总 RSD。回归分析一方面揭示了系统性差异，另一方面又揭示了系统性差异。测试和比较方法之间具有良好的相关性。从回归线周围数据的分布与总 RSD 的 95% 预测区间相比，样本相关效应和/或特异性问题显而易见并得到了研究。 (C) 2003 Elsevier B.V. 保留所有权利。
This study applied electrospray ionization-isotope dilution-liquid chromatography-tandem mass spectrometry for the evaluation of five urinary C-peptide immumoassays via split-sample measurements. The immunoassays measured in duplicate in the same run, the comparison method in triplicate over different runs. From the data, the within-run imprecision and the method comparison total RSDs were calculated. Regression analysis revealed on the one hand systematic differences, on the other. an excellent correlation between the test and comparison methods. From the spread of the data around the regression line in comparison with the 95% prediction intervals from the total RSD, sample-related effects and/or specificity problems were apparent and investigated. (C) 2003 Elsevier B.V. All rights reserved.