Maternal Deprivation Induces Depressive-like Behaviour and Alters Neurotrophin Levels in the Rat Brain
Maternal Deprivation Induces Depressive-like Behaviour and Alters Neurotrophin Levels in the Rat Brain
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DOI：
10.1007/s11064-010-0364-3
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发表时间：
2011-03
影响因子：
4.4
通讯作者：
G. Réus;R. Stringari;K. F. Ribeiro;A. Cipriano;B. Panizzutti;L. Stertz;C. Lersch;F. Kapczinski
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文献类型：
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作者：
G. Réus;R. Stringari;K. F. Ribeiro;A. Cipriano;B. Panizzutti;L. Stertz;C. Lersch;F. Kapczinski
本研究旨在评估母体剥夺对成年大鼠的行为和分子效应。为此，在成年阶段，对剥夺和非剥夺雄性大鼠进行强迫游泳和旷场测试。测定大鼠血清中促肾上腺皮质激素(ACTH)水平及前额叶皮质、海马区和杏仁核中脑源性神经营养因子(BDNF)、神经营养因子-3(NT-3)和神经生长因子(NGF)蛋白水平。我们观察到，母亲的剥夺增加了不动时间，减少了攀登时间，而不影响运动能力。母体剥夺大鼠ACTH循环水平升高。此外，与对照组相比，母体剥夺组大鼠杏仁核中BDNF蛋白水平降低，海马区和杏仁核中NT-3和NGF水平降低。总之，我们的结果支持这一观点，即在母亲剥夺模型中，行为、ACTH循环水平和神经营养因子水平的改变可能导致应激相关疾病，如抑郁症。
The present study was aimed to evaluate the behavioral and molecular effects of maternal deprivation in adult rats. To this aim, male rats deprived and non-deprived were assessed in the forced swimming and open-field tests in adult phase. In addition adrenocorticotrophin hormone (ACTH) levels was assessed in serum and brain-derived-neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and nerve growth factor (NGF) protein levels were assessed in prefrontal cortex, hippocampus and amygdala. We observed that maternal deprivation increased immobility time, and decreased climbing time, without affecting locomotor activity. ACTH circulating levels were increased in maternal deprived rats. Additionally, BDNF protein levels were reduced in the amygdala and NT-3 and NGF were reduced in both hippocampus and amygdala in maternal deprived rats, compared to control group. In conclusion, our results support the idea that behavioral, ACTH circulating levels and neurotrophins levels altered in maternal deprivation model could contribute to stress-related diseases, such as depression.