1KCa1 activity is required for cell shrinkage, phosphatidylserine translocation and death in IT lymphocyte apoptosis
1KCa1 activity is required for cell shrinkage, phosphatidylserine translocation and death in IT lymphocyte apoptosis
复制标题
DOI：
10.1038/sj.embor.embor722
复制
发表时间：
2003-02-01
期刊：
影响因子：
7.7
通讯作者：
Higgins, CE
中科院分区：
文献类型：
--
作者：
Elliott, JL;Higgins, CE
凋亡细胞体积减少（AVD）和暴露的磷脂酰丝氨酸（PtdSer）在细胞表面的细胞凋亡的早期事件。然而，负责AVD的离子通道及其与PtdSer易位和细胞死亡的关系知之甚少。钙诱导的淋巴细胞和胸腺细胞凋亡的实时分析表明，AVD发生迅速，并先于PtdSer易位。K+通道阻断剂IKCa 1可完全抑制AVD。阻断IKCa1，从而阻断AVD，也完全阻止了PtdSer易位和细胞死亡。因此，IKCa1介导的AVD是钙诱导的细胞凋亡中最早定义的必要步骤，PtdSer易位和细胞死亡都需要。
Apoptotic cell volume decrease (AVD) and exposure of phosphatidylserine (PtdSer) at the cell surface are early events in apoptosis. However, the ion channels responsible for AVD, and their relationship to PtdSer translocation and cell death are poorly understood. Real-time analysis of calcium-induced apoptosis in lymphocytes and thymocytes showed that AVD occurs rapidly, and precedes PtdSer translocation. Blockers of the K+ channel IKCa1 completely inhibited AVD. Blockade of IKCa1, and hence AVD, also completely prevented PtdSer translocation and cell death. Thus, IKCa1-mediated AVD is the earliest-defined essential step in calcium-induced apoptosis, required for both PtdSer translocation and cell death.