Injectable reactive biocomposites for bone healing in critical-size rabbit calvarial defects
Injectable reactive biocomposites for bone healing in critical-size rabbit calvarial defects
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DOI：
10.1088/1748-6041/7/2/024112
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发表时间：
2012-04-01
影响因子：
4
通讯作者：
Guelcher, Scott A.
中科院分区：
文献类型：
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作者：
Dumas, Jerald E.;BrownBaer, Pamela B.;Guelcher, Scott A.
颅面损伤可能由外伤、肿瘤消融或感染引起，并且可能需要多次手术修复。为了解决与治疗颅面骨缺陷相关的挑战，理想的材料应该能够适应复杂的缺陷（即适形），为大脑提供临时保护直至骨骼愈合，并通过输送生物制剂增强组织再生。在这项研究中，我们评估了可注射的赖氨酸衍生聚氨酯（PUR）/同种异体移植生物复合材料促进临界尺寸兔颅骨缺损骨愈合的能力。该生物复合材料表现出良好的可注射性，其特点是低屈服应力以引发材料流动，并具有高初始粘度以最大限度地减少外渗和压滤的不利现象。注射后，材料在 10-12 分钟内固化，形成坚韧的弹性固体，在愈合过程中保持机械完整性。当注射到兔子的临界尺寸颅骨缺损中时，生物复合材料支持新骨的向内生长。添加80μg·mL~(-1)重组人骨形态发生蛋白2(rhBMP-2)可增强缺损内部新骨的形成，并用新骨桥接缺损。这些观察结果表明，可注射的反应性 PUR/同种异体移植生物复合材料通过提供机械稳定性以及 rhBMP-2 的局部递送，是治疗颅骨缺损的一种有前途的方法。
Craniofacial injuries can result from trauma, tumor ablation, or infection and may require multiple surgical revisions. To address the challenges associated with treating craniofacial bone defects, an ideal material should have the ability to fit complex defects (i.e. be conformable), provide temporary protection to the brain until the bone heals, and enhance tissue regeneration with the delivery of biologics. In this study, we evaluated the ability of injectable lysine-derived polyurethane (PUR)/allograft biocomposites to promote bone healing in critical-size rabbit calvarial defects. The biocomposites exhibited favorable injectability, characterized by a low yield stress to initiate flow of the material and a high initial viscosity to minimize the adverse phenomena of extravasation and filter pressing. After injection, the materials cured within 10-12 min to form a tough, elastomeric solid that maintained mechanical integrity during the healing process. When injected into a critical-size calvarial defect in rabbits, the biocomposites supported ingrowth of new bone. The addition of 80 mu g mL(-1) recombinant human bone morphogenetic protein-2 (rhBMP-2) enhanced new bone formation in the interior of the defect, as well as bridging of the defect with new bone. These observations suggest that injectable reactive PUR/allograft biocomposites are a promising approach for healing calvarial defects by providing both mechanical stability as well as local delivery of rhBMP-2.