AN ULTRASTRUCTURAL-STUDY OF VERTICAL TRANSMISSION OF RICKETTSIA-TSUTSUGAMUSHI DURING OOGENESIS AND SPERMATOGENESIS IN LEPTOTROMBIDIUM-PALLIDUM
AN ULTRASTRUCTURAL-STUDY OF VERTICAL TRANSMISSION OF RICKETTSIA-TSUTSUGAMUSHI DURING OOGENESIS AND SPERMATOGENESIS IN LEPTOTROMBIDIUM-PALLIDUM
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DOI：
10.4269/ajtmh.1994.50.219
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发表时间：
1994-02-01
影响因子：
3.3
通讯作者：
TAMURA, A
中科院分区：
文献类型：
--
作者：
URAKAMI, H;TAKAHASHI, M;TAMURA, A
用电子显微镜观察梅毒钩端螺旋体中的立克次体，并在幼虫和成虫的不同组织器官中发现立克次体。立克次体的萌发是从寄主细胞中释放的一种方式，仅在幼虫生殖器官的雏形中观察到。卵原细胞、成熟卵母细胞和产卵后的卵均含有这些微生物。成年雄性在精原细胞、精母细胞和精子细胞中也发现立克次体，但成熟后这些细胞中的立克次体被清除。只有成熟的精子细胞，而不是消除的立克次体，迁移到睾丸的另一个无立克次体的区域，导致精子细胞与立克次体分离，并产生无立克次体的精原细胞。根据这些观察结果，立克次体向后代垂直传播的机制只发生在雌性亲本中。幼虫和成虫体细胞中的立克次体大部分为球状，但成虫卵巢和睾丸中的部分立克次体呈杆状，并被一层来源不明的膜所包裹。
Rickettsia tsutsugamushi in Leptotrombidium pallidum was observed by electron microscopy and rickettsiae were found in the various tissues and organs of both larvae and adults. Budding of rickettsiae, a manner of release from the host cells, was observed only in the rudiments of the reproductive organs in larvae. Oogonia and maturing oocytes in adult females and eggs after oviposition contained the microorganisms. In adult males, rickettsiae were also found in the spermatogonia, spermatocytes, and spermatids in the early stage of spermatogenesis, but were eliminated from these cells during maturation. Only the maturing spermatids, but not the eliminated rickettsiae, migrated to another rickettsia-free area of the testis, resulting in the separation of spermatids from rickettsiae and in the production of rickettsia-free spermatophores. Based on these observations, the mechanism of vertical transmission of the rickettsiae to the progeny occurs only in the female parents. Most rickettsiae in the somatic cells of larvae and adults were coccoid, but some rickettsiae in the ovary and the testis of adult mites showed bacillary forms and were enveloped by a membrane of unknown origin.