Effects of Antioxidant Enzymes of Ascorbate-Glutathione Cycle in Soybean (Glycine Max) Leaves Exposed to Ozone
Effects of Antioxidant Enzymes of Ascorbate-Glutathione Cycle in Soybean (Glycine Max) Leaves Exposed to Ozone
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DOI：
10.4028/www.scientific.net/amr.204-210.672
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发表时间：
2011-02
期刊：
影响因子：
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通讯作者：
T. Zhao;Jun Wang;Yan Wang;Ying Cao
中科院分区：
文献类型：
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作者：
T. Zhao;Jun Wang;Yan Wang;Ying Cao
采用开顶式气室（OTCs）研究了对流层O3胁迫下植物体内抗氧化酶清除活性氧（ROS）的机制。结果表明，与对照相比，80±10 nL·L-1和110±10 nL·L-1的O3浓度在整个生长期内均导致了丙二醛（MDA）含量的增加，超氧阴离子（O2）产生速率和过氧化氢（H2 O2）含量的降低。抗坏血酸过氧化物酶（APX）、单脱氢抗坏血酸还原酶（MDHAR）和谷胱甘肽还原酶（GR）的活性与对照相比均呈现出前期上升、后期下降的趋势，而脱氢抗坏血酸还原酶（达尔）的活性则呈现出前期上升、中期下降、后期上升的趋势。结果表明，O3浓度升高加速了活性氧（ROS）的代谢，降低了抗氧化酶的活性，使抗氧化酶不能耐受O3浓度升高引起的氧化损伤，对大豆产生伤害效应。
Open-top chambers (OTCs) were used to investigate the mechanism of antioxidant enzymes to eliminate reactive oxygen species (ROS) of plants under troposphere O3 stress. The results indicated that, compared to control, the O3 concentration of 80±10 nL·L-1 and 110±10 nL·L-1 induced an increase on malondialdehyde (MDA) content and a decrease on superoxide anion (O2)production rate and hydrogen peroxide (H2O2) content during the whole growth stage. Simultaneity, it showed a trend of increasing in earlier stage and decreasing in later stage of the activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR), while the dehydroascorbate reductase (DHAR) activity was increased in earlier period, decreased in middle periods and then increased in later period compared to control, respectively. The results show that elevated O3 concentration accelerates ROS metabolism rates, reduces the efficiency of antioxidant enzymes that can not tolerate oxidative damage caused by elevated O3 concentration, which represents injured affects to soybean.