Integrated quantitative analysis of nitrogen stress response in Chlamydomonas reinhardtii using metabolite and protein profiling.
Integrated quantitative analysis of nitrogen stress response in Chlamydomonas reinhardtii using metabolite and protein profiling.
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DOI：
10.1021/pr400952z
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发表时间：
2014-02
影响因子：
4.4
通讯作者：
Nishikant Wase;P. Black;B. Stanley;C. DiRusso
中科院分区：
文献类型：
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作者：
Nishikant Wase;P. Black;B. Stanley;C. DiRusso
氮饥饿诱导微藻的整体应激反应，导致脂质作为生物燃料的潜在来源的积累。利用基于GC-MS的代谢产物和iTRAQ标记的蛋白质谱分析，我们研究并关联了氮胁迫下莱茵衣藻的代谢和蛋白质组学反应。参与氮保留途径、甲基转移反应和能量产生的关键氨基酸和代谢物的丰度减少，而某些脂肪酸、柠檬酸、蛋氨酸、柠檬酸苹果酸、三乙醇胺、烟酰胺、海藻糖和山梨醇的丰度增加。与非应激培养物相比，参与氮同化、氨基酸代谢、氧化磷酸化、糖酵解、TCA循环、淀粉和脂质代谢的蛋白质升高。相反，乙醛酸循环、一碳代谢、戊糖磷酸途径、卡尔文循环、光合和捕光复合物以及核糖体的酶减少。一个值得注意的观察结果是，在氮胁迫过程中积累的柠檬酸盐与产生或利用这种代谢产物的酶的变化相协调，证明了比较蛋白质和代谢产物谱以了解代谢流的复杂模式的价值。因此，目前的研究提供了独特的洞察全球代谢调整，导致脂质储存在N饥饿的应用对先进的生物燃料生产技术。
Nitrogen starvation induces a global stress response in microalgae that results in the accumulation of lipids as a potential source of biofuel. Using GC-MS-based metabolite and iTRAQ-labeled protein profiling, we examined and correlated the metabolic and proteomic response of Chlamydomonas reinhardtii under nitrogen stress. Key amino acids and metabolites involved in nitrogen sparing pathways, methyl group transfer reactions, and energy production were decreased in abundance, whereas certain fatty acids, citric acid, methionine, citramalic acid, triethanolamine, nicotianamine, trehalose, and sorbitol were increased in abundance. Proteins involved in nitrogen assimilation, amino acid metabolism, oxidative phosphorylation, glycolysis, TCA cycle, starch, and lipid metabolism were elevated compared with nonstressed cultures. In contrast, the enzymes of the glyoxylate cycle, one carbon metabolism, pentose phosphate pathway, the Calvin cycle, photosynthetic and light harvesting complex, and ribosomes were reduced. A noteworthy observation was that citrate accumulated during nitrogen stress coordinate with alterations in the enzymes that produce or utilize this metabolite, demonstrating the value of comparing protein and metabolite profiles to understand complex patterns of metabolic flow. Thus, the current study provides unique insight into the global metabolic adjustments leading to lipid storage during N starvation for application toward advanced biofuel production technologies.