Quantitative proteomic analysis of mice corneal tissues reveals angiogenesis-related proteins involved in corneal neovascularization
Quantitative proteomic analysis of mice corneal tissues reveals angiogenesis-related proteins involved in corneal neovascularization
复制标题
小鼠角膜组织的定量蛋白质组学分析揭示了参与角膜新生血管形成的血管生成相关蛋白
DOI：
10.1016/j.bbapap.2016.04.001
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发表时间：
2016-07-01
影响因子：
3.2
通讯作者：
Zhou, Hu
中科院分区：
文献类型：
--
作者：
Shen, Minqian;Tao, Yimin;Zhou, Hu
Corneal neovascularization (CNV) was induced in Balb/c mice by alkali burns in the central area of the cornea with a diameter of 2.5 mm. After fourteen days, the cornea from one eye was collected for histological staining for CNV examination, while the cornea from the other eye of the same mouse was harvested for proteomic analysis. The label-free quantitative proteomic approach was applied to analyze five normal corneal tissues (normal group mice n = 5) and five corresponding neovascularized corneal tissues (model group mice n = 5). A total of 2124 proteins were identified, and 1682 proteins were quantified from these corneal tissues. Among these quantified proteins, 290 proteins were significantly changed between normal and alkali burned corneal tissues. Of these significantly changed proteins, 35 were reported or predicted as angiogenesis-related proteins. Then, these 35 proteins were analyzed using Ingenuity Pathway Analysis Software, resulting in 26 proteins enriched and connected,to each other in the protein-protein interaction network, such as Lcn-2, alpha B-crystallin and Serpinf1 (PEDF). These three significantly changed proteins were selected for further Western blotting validation. Consistent with the quantitative proteomic results, Western blotting showed that Lcn-2 and aB-crystallin were significantly up-regulated in CNV model, while PEDF was down-regulated. This study provided increased understanding of angiogenesis-related proteins involved in corneal vascular development, which will be useful in the ophthalmic clinic of specifically target angiogenesis. (C) 2016 Elsevier B.V. All rights reserved.
