Histones bind more tightly to bromodeoxyuridine-substituted DNA than to normal DNA.
Histones bind more tightly to bromodeoxyuridine-substituted DNA than to normal DNA.
复制标题
组蛋白与溴脱氧尿苷取代的 DNA 的结合比与正常 DNA 的结合更紧密。
作者：
S. Lin;D. Lin;A. Riggs
使用膜过滤测定，我们从动力学和竞争实验中获得了结果，表明组蛋白与溴脱氧尿苷取代的 DNA 的结合比与正常 DNA 的结合更强。在 37°C 的 0.2 M 离子强度标准缓冲液中，组蛋白 H1、H2 和 h4 从 BrdU 取代的 DNA 中解离的速率分别比从正常 DNA 中解离的速率慢 7、4 和 2 倍。竞争实验表明，BrdU 取代的 DNA 和正常 DNA 在组蛋白结合方面存在更大的差异。组蛋白与 BrdU 取代的 DNA 的更紧密结合引起了人们的兴趣，因为已知 BrdU 对真核染色体凝聚和染色、病毒诱导和分化抑制的影响。
Using a membrane filter assay, we have obtained results from both kinetic and competition experiments indicating that histones bind more strongly to bromodeoxyuridine-substituted DNA than to normal DNA. At 37 degrees C in our standard buffer of 0.2 M ionic strength, the rate of dissociation of histones H1, H2, and h4 from BrdU-substituted DNA is respectively 7, 4, and 2 times slower than it is from normal DNA. Competition experiments show an even greater difference between BrdU-substituted and normal DNA with respect to histone binding. The tighter binding of histones to BrdU-substituted DNA is of interest because of the known effects of BrdU on eukaryotic chromosome condensation and staining, virus induction, and the inhibition of differentiation.