Fractionation of Mutagens in Chlorinated Tap Water and Their Reactivity with Sulfite.
Fractionation of Mutagens in Chlorinated Tap Water and Their Reactivity with Sulfite.
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氯化自来水中诱变剂的分级及其与亚硫酸盐的反应性。
DOI：
10.2965/jswe.15.814
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
T. Matsuo
中科院分区：
文献类型：
--
作者：
N. Suzuki;J. Nakanishi;T. Matsuo
对氯化自来水的诱变提取液进行了高效液相色谱分离。在pH值为2的条件下，用XAD-4吸附浓缩约19000l的氯化自来水，然后用二氯甲烷洗脱。这种浓缩物分为酸性和中性两种馏分。每一组分均经反相高效液相色谱分离。对每一组分的HPLC亚组份进行致突变性检测，并分析强致突变剂3-chloro-4-dichloromethy-5-hydroxy-2(5H)-furanone(MX)。酸性组分中只有两个亚组分具有显著的致突变性，其中一个亚组分含有MX，这解释了该组分55%的致突变性。另一个诱变亚组分不含MX。这些亚组分中的未知诱变剂可能在结构或化学性质上与MX有相似之处，中性组分的高效液相色谱分离产生了许多诱变亚组分。这意味着中性组分含有许多诱变剂，这些诱变剂可能与MX不同。该组分中的诱变剂比酸性组分具有更多的未知性质，并研究了诱变剂与亚硫酸盐的反应活性。在1.5氯当量的亚硫酸盐作用6.5h后，自来水的致突变性被破坏了约60%。用相当于5.0氯气浓度的亚硫酸盐作用28h，诱变剂的杀灭率为76%。
The mutagenic extract of chlorinated tap water was subjected to HPLC fractionation. About 19,000l of chlorinated tap water was concentrated by XAD-4 absorption in pH 2, followed by the elution by dichloromethane. This concentrate was divided into acidic and neutral fractions. Each fraction was subjected to ODS-reversed phase HPLC fractionation. HPLC subfractions of each fraction were assayed for mutagenicity and analyzed for the strong mutagen, 3-chloro-4-dichloromethy-5-hydroxy-2(5H)-furanone (MX). Only two of nine HPLC subfractions from the acidic fraction showed significant mutagenicity, and one subfraction contained MX, which explained 55% mutagenicity of that subtraction. Another mutagenic subfraction did not contain MX. Unknown mutagens in these subfractions were presumed to have similarities to MX in their structures or chemical natures.HPLC fractionation of neutral fraction resulted in many mutagenic subfractions. This implies that the neutral fraction contained many mutagens, which might not be similar to MX. Mutagens in this fraction have more unknown nature than the acidic fraction.Reactivity of the mutagens and sulfite was also studied. About 60% of the mutagenicity of the tap water was destroyed after 6.5h reaction with sulfite in 1.5 chlorine equivalent concentration. 28h reaction with sulfite in 5.0 chorine equivalent concentration destroyed 76% mutagens.