Phylogenetic Analysis of the Hemagglutinin Genes of 12 H9N2 Influenza Viruses Isolated from Chickens in Iran from 2003 to 2005
Phylogenetic Analysis of the Hemagglutinin Genes of 12 H9N2 Influenza Viruses Isolated from Chickens in Iran from 2003 to 2005
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DOI：
10.1637/9103-101309-reg.1
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发表时间：
2010-06-01
期刊：
影响因子：
1.4
通讯作者：
Ghorbani, A.
中科院分区：
文献类型：
--
作者：
Moosakhani, F.;Shoshtari, A. H.;Ghorbani, A.
本研究从2003年至2005年从伊朗不同省份的鸡群中分离到12株H9N2亚型流感病毒的血凝素基因，并对其进行扩增和测序。所有的12个分离株在裂解位点RSSF/GLF上都有相似的序列，含有8个潜在的糖基化位点，并在受体结合部位具有共同的特征氨基酸残基丙氨酸-190、谷氨酰胺-226和谷氨酰胺-227。在这12个分离株中，有10个在226位含有亮氨酸，这在人类H2和H3株中发现的序列中占主导地位。总体而言，在这些伊朗家禽中存在已知与人型受体结合的序列，并在伊朗人群中存在对H9N2的抗体，这表明在伊朗传播的H9N2型禽流感病毒有可能感染人类。因此，强烈建议在这个国家广泛监测H9N2。
In the present study, the hemagglutinin genes from 12 influenza viruses of the H9N2 subtype were isolated from chicken flocks in different provinces of Iran from 2003 to 2005, amplified and sequenced. All of the 12 isolates showed similar sequences at the cleavage site, RSSF/GLF, bearing eight potential glycosylation sites and sharing the characteristic deduced amino acid residues alanine-190, glutamine-226, and glutamine-227 at the receptor-binding site. Ten out of these 12 isolates possessed leucine at position 226, which prevails in the sequences found in human H2 and H3 strains. Overall, the presence in these Iranian poultry H9N2 viruses of the sequence known to bind to human-type receptors and the presence of antibodies in the human population of Iran to H9N2 showed that it is possible for circulating H9N2 avian influenza viruses in Iran to infect humans. Hence, extensive surveillance of H9N2 in this country is highly recommended.