Comparative proteomic analyses reveal the changes of metabolic features in soybean (Glycine max) pistils upon pollination
Comparative proteomic analyses reveal the changes of metabolic features in soybean (Glycine max) pistils upon pollination
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DOI：
10.1007/s00497-012-0197-0
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发表时间：
2012-12-01
影响因子：
--
通讯作者：
Yang, Pingfang
中科院分区：
文献类型：
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作者：
Li, Ming;Sha, Aihua;Yang, Pingfang
管配子是植物生殖生长的关键过程，在此过程中，雌蕊和花粉之间发生了大量的细胞-细胞相互作用事件。以前在茄科、罂粟科和十字花科的研究集中于自交不亲和系统中的花粉-柱头识别，提供了许多重要的观点。在本研究中，我们对大豆成熟雌蕊授粉前后的蛋白质组进行了分析。对未授粉和已授粉雌蕊的双向凝胶电泳图进行了比较分析。结果表明，授粉后22个蛋白质增加，36个蛋白质减少。功能分类表明，它们大多是代谢和氧化还原相关的蛋白质。加强初级代谢，促进花粉管导向化合物的生物合成，调节氧化还原动态平衡系统，可能有助于授粉成功。定量逆转录-聚合酶链式反应分析表明，授粉过程中基因表达的调控可能发生在转录和转录后水平。这项研究将加深我们对花粉-柱头在植物有性生殖生长中相互作用的理解。
Siphonogamy is a critical process in plant reproductive growth, during which numerous cell-cell interaction events occur between pistil and pollen. Previous studies in Solanaceae, Papaveraceae, and Brassicaceae focusing on pollen-stigma recognition in self-incompatible systems have provided many important views. In this study, we profiled the proteome in soybean mature pistils before and after pollination. Comparative analyses of two-dimensional gel electrophoresis maps from un-pollinated and pollinated pistils were conducted. The results showed that 22 proteins were increased and 36 proteins decreased after pollination. Functional categorization showed that most of them were metabolism- and redox-related proteins. The enhancement of primary metabolism, biosynthesis of pollen tube guidance compounds, and adjustment of redox homeostasis system might be helpful for a successful pollination. Quantitative reverse transcript-polymerase chain reaction analysis implied that the regulation of gene expression might happen at both transcriptional and posttranscriptional levels during pollination. This study will enhance our understanding of pollen-stigma interaction in plant sexual reproductive growth.