A description of the technique for nuclear transplantation in Xenopus laevis.
A description of the technique for nuclear transplantation in Xenopus laevis.
复制标题
非洲爪蟾核移植技术的描述。
DOI：
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发表时间：
1960
期刊：
影响因子：
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通讯作者：
M. Fischberg
中科院分区：
文献类型：
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作者：
T. Elsdale;J. Gurdon;M. Fischberg
A Method by which nuclei can be successfully transplanted into Amphibian eggs was first worked out by Briggs & King (1952) for the eggs of Rana pipiens . Xenopus laevis is an atypical Anuran since its eggs can be obtained throughout the year, and the resulting embryos can be reared to maturity within 12 months under laboratory conditions. Because of these advantages we have used Xenopus for nuclear transplantation experiments. Though the principle of Briggs & King9s technique has been followed, differences between the eggs of Rana and Xenopus have made it necessary to modify their technique before it can be satisfactorily applied to the eggs of Xenopus . The purpose of this publication is first to give technical details of these modifications, and secondly to discuss the extent to which they might affect a direct comparison of the results of transplantations in Rana and Xenopus .
