AN IMMUNOHISTOCHEMICAL STUDY OF THE DISTRIBUTION OF IMMUNOCOMPETENT CELLS, ESPECIALLY MACROPHAGES AND IA ANTIGEN-EXPRESSING CELLS OF HETEROGENEOUS POPULATIONS, IN NORMAL RAT MOLAR PULP
AN IMMUNOHISTOCHEMICAL STUDY OF THE DISTRIBUTION OF IMMUNOCOMPETENT CELLS, ESPECIALLY MACROPHAGES AND IA ANTIGEN-EXPRESSING CELLS OF HETEROGENEOUS POPULATIONS, IN NORMAL RAT MOLAR PULP
复制标题
DOI：
10.1177/00220345920710051201
复制
发表时间：
1992-05-01
影响因子：
7.6
通讯作者：
SUDA, H
中科院分区：
文献类型：
--
作者：
OKIJI, T;KAWASHIMA, N;SUDA, H
用7种抗鼠单抗进行免疫组织化学染色，观察各种免疫活性细胞在大鼠磨牙髓内的精确分布。结果表明，大鼠磨牙髓内含有大量的OX6(抗Ia抗原)阳性细胞和大量ED1(抗单核细胞、巨噬细胞和树突状细胞)阳性细胞、ED2(抗组织巨噬细胞)阳性细胞和/或OX35(抗巨噬细胞和CD4+淋巴细胞)阳性细胞。巨噬细胞样细胞主要分布在牙髓中央，而树突状细胞多见于牙髓周围。免疫组织化学双重染色显示，这些细胞具有一定的异质性，但大多数为ED1+/OX6-/ED2+细胞，可能为组织细胞。研究结果还表明，真正的树突状细胞可能属于ED1+/OX6+/ED2-细胞类别。还检测到少量T淋巴细胞和浆细胞。这些结果表明，正常牙髓中含有多种免疫活性细胞，其中以巨噬细胞为主。在外源性致病刺激侵入牙髓后，这些细胞可能通过吞噬细胞或抗原提呈细胞参与防御反应，而吞噬细胞或抗原提呈细胞是启动免疫反应的关键。
The precise distribution of various immunocompetent cells in rat molar pulp was immunohistochemically examined by use of seven anti-rat monoclonal antibodies. It was demonstrated that rat molar pulp contained many OX6 (anti-Ia antigen)-positive cells and a large number of ED1 (anti-monocytes, macrophages, and dendritic cells)-positive, ED2 (anti-tissue macrophages)-positive, and/or OX35 (anti-macrophages and CD4+ lymphocytes)-positive cells. Macrophage-like cells predominated in the central portion of the pulp, while cells of dendritic appearance usually existed in the periphery of the pulp. Double-immunoperoxidase staining revealed that these cells showed some heterogeneity, but the majority could be classified as ED1+/OX6-/ED2+ cells, which may be la- histiocytes. Findings also suggested that true dendritic cells may be included in the ED1+/OX6+/ED2- category of cells. A small number of T lymphocytes and plasma cells were also detected. These results suggest that the normal dental pulp contains a variety of immunocompetent cells, with macrophages as the most dominating. Following the exogenous invasion of pathogenic stimuli in the pulp, these cells may participate in the defense reaction by acting as phagocytes or antigen-presenting cells, which are essential for the initiation of immune responses.