Column efficiency in micellar electrokinetic capillary chromatography
Column efficiency in micellar electrokinetic capillary chromatography
复制标题
胶束电动毛细管色谱中的柱效率
DOI：
10.1021/ac00130a020
复制
发表时间：
1987
影响因子：
7.4
通讯作者：
R. Cole
中科院分区：
文献类型：
--
作者：
M. Sepaniak;R. Cole
研究了影响胶束电动毛细管色谱柱效率的实验因素。研究发现，施加电压、色谱柱尺寸、流动相中缓冲液和表面活性剂的浓度等参数都会影响效率。范德姆特样图的板与施加电压是用来证明因素的重要性，通常导致带分散在色谱和电泳。由于流动相的传质阻力和柱内的温度梯度引起的色散是最显著的。板材高度小于10..亩。当实验参数优化时，M是可能的。
Experimental factors that influence column efficiency in micellar electrokinetic capillary chromatography were studied. Parameters, such as applied voltage, column dimensions, and concentrations of buffer and surfactant in the mobile phase, are found to influence efficiency. Van Deemter-like plots of plate vs. applied voltage are used to demonstrate the significance of factors which typically cause band dispersion in chromatography and electrophoresis. Dispersion due to resistance to mass transfer in the mobile phase and temperature gradients within the column are shown to be most significant. Plate heights of less than 10..mu..m are possible when experimental parameters are optimized.