FERRITIN SYNTHESIS AND IRON UPTAKE IN DEVELOPING ERYTHROID-CELLS
FERRITIN SYNTHESIS AND IRON UPTAKE IN DEVELOPING ERYTHROID-CELLS
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DOI：
10.1002/ajh.2830060409
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发表时间：
1979-01-01
影响因子：
12.8
通讯作者：
IZAK, G
中科院分区：
文献类型：
--
作者：
KONIJN, AM;HERSHKO, C;IZAK, G
在体外研究了经放线菌素D同步化的兔骨髓细胞成熟对红系前体细胞铁蛋白合成和铁摄取的影响。早期单核细胞前体细胞与嗜碱性正常成红细胞和网织红细胞进行比较。脱辅基铁蛋白合成测定以下掺入纯化的铁蛋白的3 H-氨基酸，这是与总蛋白质合成和放射性铁的吸收全细胞和放射性铁掺入血红素和铁蛋白。随着细胞成熟度的增加，观察到脱铁铁蛋白和总蛋白合成的逐步减少，未分化细胞合成的蛋白质是网织红细胞的12倍，铁蛋白是网织红细胞的20倍。铁蛋白的合成率呈负相关的放射性铁的摄取率由全细胞和放射性铁纳入血红素;最低的放射性摄取率和血红素合成率被发现在未分化的细胞和最高的率在网织红细胞。铁蛋白中的放射性铁掺入嗜碱性正常成红细胞高于未分化细胞，但在网织红细胞中非常低。在发育中的红系细胞中，最大铁蛋白合成的阶段明显先于最大铁摄取的阶段。新形成的脱铁铁蛋白的积累，其次是增加细胞铁的吸收和铁蛋白中的铁保留增加。随着细胞持续成熟，铁蛋白Fe和直接来源于转铁蛋白的Fe用于Hb合成。
The effect of maturation on ferritin synthesis and Fe uptake by erythroid precursor cells was studied in vitro in rabbit bone marrow cells synchronized by actinomycin D. Early mononuclear precursors were compared with basophilic normoblasts and reticulocytes. Apoferritin synthesis was measured by following incorporation of 3H-amino acids into purified ferritin and this was correlated with total protein synthesis and radioactive Fe uptake by whole cells and radioactive Fe incorporation into heme and ferritin. With increasing cellular maturation, progressive reduction in apoferritin and total protein synthesis was observed, undifferentiated cells synthesizing 12 times more protein and 20 times more ferritin than reticulocytes. The rate of ferritin synthesis was inversely related to the rate of radioactive Fe uptake by whole cells and radioactive Fe incorporation into heme; lowest rates of radioactive uptake and heme synthesis were found in undifferentiated cells and highest rates in reticulocytes. Radioactive Fe incorporation into ferritin was higher in basophilic normoblasts than in undifferentiated cells but was very low in reticulocytes. In developing erythroid cells the phase of maximal ferritin synthesis apparently precedes the phase of maximal Fe uptake. Accumulation of newly formed apoferritin was followed by increase in cellular Fe uptake and increased retention of Fe in ferritin. With continuing cellular maturation, ferritin Fe and Fe derived directly from transferrin are utilized for Hb synthesis.