CROSS-IDIOTYPIC SPECIFICITY AMONG MONOCLONAL IGM PROTEINS WITH ANTI-GAMMA-GLOBULIN ACTIVITY
CROSS-IDIOTYPIC SPECIFICITY AMONG MONOCLONAL IGM PROTEINS WITH ANTI-GAMMA-GLOBULIN ACTIVITY
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DOI：
10.1084/jem.137.2.331
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发表时间：
1973-01-01
影响因子：
15.3
通讯作者：
CAPRA, JD
中科院分区：
文献类型：
--
作者：
KUNKEL, HG;AGNELLO, V;CAPRA, JD
通过使用针对单个分离的IgM抗γ球蛋白单克隆抗体制备的吸收独特型抗血清，证明了与其他IgM抗γ球蛋白的部分交叉独特型特异性。这种抗血清将这些蛋白质分为至少三组。包括60%的抗-γ-球蛋白的主要组特别均一。血凝系统和血凝抑制系统对抗γ球蛋白特异性抗原的检测效果最好。在缺乏抗γ-球蛋白活性的单克隆IgM蛋白中未发现它们，尽管在混合免疫球蛋白制备物以及异质性抗Rh抗体中检测到低浓度的相关抗原，但获得了几条证据，表明抗体结合位点参与了特异性决定簇。尝试分析每种抗-γ-球蛋白对人免疫球蛋白不同亚类Fc片段以及其他种属Fc片段的精细特异性。观察到了差异，但这些差异与交叉特异性抗原无关，抗γ球蛋白特异性抗原与先前描述的单克隆IgM冷凝集素非常相似。虽然每种蛋白质都可以根据个体独特型抗原与所有其他蛋白质区分开来，但具有这些活性的特定蛋白质组的共同抗原是突出的，并且容易用多种抗血清检测到。结果表明，即使在不相关的个体中，给定活性的蛋白质之间也存在基本相似性。
Through the use of absorbed idiotypic antisera prepared against single isolated monoclonal IgM anti-γ-globulins, partial cross-idiotypic specificity was demonstrated with other IgM anti-γ-globulins. Such antisera classified these proteins into at least three groups. The major group which included 60% of the anti-γ-globulins was particularly homogeneous. The anti-γ-globulin specific antigens were detected best in hemagglutination and hemagglutination inhibition systems. They were not found in monoclonal IgM proteins that lacked anti-γ-globulin activity although related antigens were detected at low concentrations in pooled immunoglobulin preparations as well as in heterogeneous anti-Rh antibodies.Several lines of evidence were obtained indicating that the antibody combining site was involved in the specific determinants. Attempts were made to analyze the fine specificity of each anti-γ-globulin for the Fc fragment of different subclasses of human immunoglobulins as well as those of other species. Differences were observed but these were not readily related to the cross-specificity antigens.The anti-γ-globulin specific antigens were very analogous to those previously described for monoclonal IgM cold agglutinins. Although each protein could be distinguished from all the others on the basis of individual idiotypic antigens, the antigens common to the specific groups of proteins with each of these activities were prominent and readily detected with multiple antisera. The results indicate basic similarities between proteins of a given activity even in unrelated individuals.