A macrophage-dependent factor that stimulates the proliferation of fibroblasts in vitro.
A macrophage-dependent factor that stimulates the proliferation of fibroblasts in vitro.
复制标题
一种巨噬细胞依赖性因子，可在体外刺激成纤维细胞增殖。
作者：
S. Leibovich;R. Ross
全血清（HBS）在体外刺激成纤维细胞增殖，而贫血小板血浆（PPPS）则不然。在 PPPS 存在下生长的成纤维细胞是真正静止的，因为它们不会剥夺培养基中的营养，并且只有不到 3% 的细胞合成 DNA 并分裂。体内实验表明，巨噬细胞对于伤口修复过程中刺激纤维增生是必需的。我们利用 HBS 和 PPPS 之间促生长活性的差异来研究巨噬细胞在细胞培养中产生促生长活性的能力。体外培养的豚鼠腹膜巨噬细胞在含有PPPS的培养基中直接或间接地向培养基中释放一种刺激豚鼠伤口成纤维细胞增殖的因子。这种巨噬细胞依赖性成纤维细胞刺激活性 (MFSA) 是不可透析的、热稳定的（56°C 30 分钟），并且需要体外培养来证明活性。 MFSA 与其他生长因子之间的关系尚未确定。与巨噬细胞相反，从肠系膜淋巴结制备的淋巴细胞不产生成纤维细胞刺激活性。
Whole blood serum (HBS) stimulates the proliferation of fibroblasts in vitro, while platelet-poor plasma serum (PPPS) does not. Fibroblasts grown in the presence of PPPS are truly quiescent in that they are not deprived of nutrients in the culture medium and less than 3% of the cells synthesize DNA and divide. In vivo experiments have suggested that macrophages are necessary for stimulation of fibroplasia during wound repair. We have utilized the difference in growth-promoting activity between HBS and PPPS to study the ability of macrophages to produce growth-promoting activity in cell culture. Guinea pig peritoneal macrophages cultured in vitro in medium containing PPPS release into the medium, either directly or indirectly, a factor (or factors) that stimulates the proliferation of guinea pig wound fibroblasts. This macrophage-dependent, fibroblast-stimulating activity (MFSA) is nondialyzable, heat stable (56 C for 30 minutes), and requires culture in vitro for demonstration of activity. The relationship between MFSA and other growth factor(s) has not yet been determined. In contrast to the macrophage, lymphocytes prepared from mesenteric lymph nodes produced no figroblast-stimulating activity.