Albumin and fibrinogen adsorption on Cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly(hydroxyethylmethacrylate)) surfaces
Albumin and fibrinogen adsorption on Cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly(hydroxyethylmethacrylate)) surfaces
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DOI：
10.1163/156856203766652057
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发表时间：
2003-01
期刊：
影响因子：
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通讯作者：
M. Martins;D. Wang;J. Ji;L. Feng;M. Barbosa
中科院分区：
文献类型：
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作者：
M. Martins;D. Wang;J. Ji;L. Feng;M. Barbosa
本研究旨在研究Cibacron蓝F3G-A （CB）在PU-PHEMA（聚氨酯-聚（羟乙基甲基丙烯酸酯））表面固定化对蛋白质吸附和细菌粘附的影响。通过三嗪环与聚合物羟基之间的共价键来固定CB。通过衰减全反射傅里叶变换红外光谱（ATR-FT-IR）、接触角测量、x射线光电子能谱（XPS）和扫描电子显微镜（SEM）对薄膜进行了表征。用放射性标记白蛋白和纤维蛋白原从纯溶液、两者的混合物和血浆中评估CB效率。同时进行了白蛋白预涂前后的细菌粘附试验。CB的存在增加了白蛋白和纤维蛋白原在PU-PHEMA表面的吸附。CB与PU-PHEMA表面的结合也增加了细菌的粘附性。尽管白蛋白预涂层降低了细菌在PU和PU- phema - cb表面的粘附力（分别降低67%和80%），但细菌在PU和PU- phema表面的粘附力始终低于PU- phema - cb表面。这些结果表明，与已描述的与葡聚糖结合的CB相比，CB固定在PU- phema表面对白蛋白的选择性较低，并且相对于PU和PU- phema表面增加了细菌的粘附。
In the present work, it is intended to study the effect of Cibacron blue F3G-A (CB) immobilised onto PU-PHEMA (polyurethane-poly(hydroxyethylmethacrylate)) surfaces on protein adsorption and bacterial adhesion. CB immobilisation was carried out by covalent binding between its triazine ring and the hydroxyl groups of the polymer. Characterisation of the films was carried out by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT-IR), contact angle measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). CB efficiency was evaluated using radiolabelled albumin and fibrinogen from pure solutions, mixtures of both and plasma. Bacterial adhesion tests before and after albumin pre-coating were also performed. The presence of CB increases albumin and fibrinogen adsorption to PU-PHEMA surfaces. The incorporation of CB onto the PU-PHEMA surface also increases bacterial adhesion. Although albumin pre-coating decreases bacterial adhesion onto PU (67% decrease) and PU-PHEMA-CB (80%), bacterial adhesion is always lower on PU and PU-PHEMA surfaces than on PU-PHEMA-CB. These results demonstrate that, in contrast to what has been described for CB bound to dextran, CB immobilisation on PU-PHEMA surfaces presents low selectivity to albumin and increased bacterial adhesion relatively to PU and PU-PHEMA surfaces.