The anchor cell initiates dorsal lumen formation during C. elegans vulval tubulogenesis
The anchor cell initiates dorsal lumen formation during C. elegans vulval tubulogenesis
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DOI：
10.1016/j.ydbio.2009.01.034
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发表时间：
2009-04-15
影响因子：
2.7
通讯作者：
Hanna-Rose, Wendy
中科院分区：
文献类型：
--
作者：
Estes, Kathleen A.;Hanna-Rose, Wendy
小管形成和管腔形成对大多数器官的发育至关重要。我们研究秀丽隐杆线虫外阴和子宫的发展，以探讨复杂的机制，介导这些事件。外阴和腹侧子宫的发育由称为锚细胞（AC）的性腺细胞的诱导细胞信号传导活动协调。我们证明，除了它的功能，在指定的命运，AC直接促进背外阴[ tubulogenesis。具有缺陷锚细胞行为的两种类型的突变体揭示了外阴的锚细胞侵入对于形成背侧外阴细胞的环形形状是重要的，vulF在fos-1突变体中，AC不能破坏性腺和外阴之间的基底膜，在unc-6 netrin或其受体unc-40的突变体中，这导致AC迁移缺陷，AC不能侵入外阴，外阴无管腔形成。通过研究背侧外阴细胞命运的GFP标记，我们证明了命运规范缺陷并不能解释异常的外阴形状。我们认为，AC的存在下，在中央的发展外阴环所需的背侧外阴腔的形成，以完成外阴tubulogenesis。(C)2009爱思唯尔公司All rights reserved.
Tubulogenesis and lumen formation are critical to the development of most organs. We study Caenorhabditis elegans vulval and uterine development to probe the complex mechanisms that mediate these events. Development of the vulva and the ventral uterus is coordinated by the inductive cell-signaling activity of a gonadal cell called the anchor cell (AC). We demonstrate that in addition to its function in specifying fate, the AC directly promotes dorsal vulva[ tubulogenesis. Two types of mutants with defective anchor cell behavior reveal that anchor cell invasion of the vulva is important for forming the toroidal shape of the dorsal vulval cell, vulF In fos-1 mutants, where the AC cannot breakdown the basement membranes between the gonad and the vulva, and in mutants in unc-6 netrin or its receptor unc-40, which cause AC migration defects, the AC fails to invade the vulva and no lumen is formed in vulF. By examining GFP markers of dorsal vulval cell fate, we demonstrate that fate specification defects do not account for the aberrant vulF shape. We propose that the presence of the AC in the center of the developing vulF toroid is required for dorsal vulval lumen formation to complete vulval tubulogenesis. (C) 2009 Elsevier Inc. All rights reserved.