Targeted nuclear antisense RNA mimics natural antisense-induced degradation of polyoma virus early RNA.
Targeted nuclear antisense RNA mimics natural antisense-induced degradation of polyoma virus early RNA.
复制标题
靶向核反义 RNA 模拟自然反义诱导的多瘤病毒早期 RNA 降解。
DOI：
10.1073/pnas.91.10.4258
复制
发表时间：
1994
影响因子：
11.1
通讯作者：
Carmichael,GG
中科院分区：
文献类型：
--
作者：
Liu,Z;Batt,DB;Carmichael,GG
我们描述了抑制靶基因表达的通用反义策略。在反义表达载体中用顺式作用核酶替代聚腺苷酸化信号会导致 RNA 保留在核中并有效降解其靶标。我们证明了该系统在多瘤病毒中的实用性，其中早期链 RNA 水平在细胞核中被反义晚期链对应物下调。我们发现，破坏这些自然发生的反义转录本稳定性的突变会导致早期链 RNA 水平增加。此外，核反义转录本的反式表达降低了早期链RNA水平并定量模拟了自然调节。
We describe a general antisense strategy to inhibit target gene expression. The substitution of a cis-acting ribozyme for a polyadenylylation signal in an antisense expression vector results in the nuclear retention of RNAs and the efficient degradation of their targets. We demonstrate the utility of this system in polyoma virus, where early-strand RNA levels are downregulated in the nucleus by antisense late-strand counterparts. We show that mutations destabilizing these naturally occurring antisense transcripts lead to increased levels of early-strand RNAs. Furthermore, expression in trans of nuclear antisense transcripts lowers early-strand RNA levels and quantitatively mimics the natural regulation.