YGL138(t), encoding a putative signal recognition particle 54 kDa protein, is involved in chloroplast development of rice.
YGL138(t), encoding a putative signal recognition particle 54 kDa protein, is involved in chloroplast development of rice.
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YGL138(t)，编码假定的信号识别颗粒 54 kDa 蛋白，参与水稻叶绿体发育
DOI：
10.1186/1939-8433-6-7
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发表时间：
2013-03-27
期刊：
影响因子：
--
通讯作者：
Xie J
中科院分区：
文献类型：
--
作者：
Zhang F;Luo X;Hu B;Wan Y;Xie J
背景叶绿体的正常发育对植物至关重要，但其生物学机制仍不完全清楚，特别是在水稻中。经甲基磺酸乙酯（EMS）处理后，分离得到一株日本亚种（ssp.japonica）。该突变体在整个发育过程中表现出明显的黄绿叶表型，叶绿素水平降低，叶绿体发育受阻。Theygl138突变体的表型是由一个核基因引起的，初步设计为YGL138（t）。YGL138（t）基因定位于第11染色体上，通过图位克隆将其定位于91.8kb的区域内。Os11g05552基因编码信号识别颗粒54 kDa（SRP54）蛋白，是一个叶绿体前体蛋白。Os11g05552基因在ygl138编码区缺失18 bp，导致编码区发生移码。结论本研究结果对进一步研究YGL138（t）基因和阐明SRP54蛋白参与水稻叶绿体发育的机制具有重要意义。
BackgroundNormal development of chloroplast is vitally important to plants, but its biological mechanism is still far from fully being understood, especially in rice.ResultsIn this study, a novel yellow-green leaf mutant,ygl138, derived from Nipponbare (Oryza sativaL. ssp.japonica) treated by ethyl methanesulfonate (EMS), was isolated. The mutant exhibited a distinct yellow-green leaf phenotype throughout development, reduced chlorophyll level, and arrested chloroplast development. The phenotype of theygl138mutant was caused by a single nuclear gene, which was tentatively designed asYGL138(t). TheYGL138(t) locus was mapped to chromosome 11 and isolated into a confined region of 91.8 kb by map-based cloning. Sequencing analysis revealed that,Os11g05552, which was predicted to encode a signal recognition particle 54 kDa (SRP54) protein and act as a chloroplast precursor, had 18 bp nucleotides deletion in the coding region ofygl138and led to a frameshift. Furthermore, the identity ofOs11g05552was verified by transgenic complementation.ConclusionsThese results are very valuable for further study onYGL138(t) gene and illuminating the mechanism of SRP54 protein involving in chloroplast development of rice.