Adipogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice - including relationship of sex differences
Adipogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice - including relationship of sex differences
复制标题
DOI：
10.1016/j.bbrc.2004.05.021
复制
发表时间：
2004-06-25
影响因子：
3.1
通讯作者：
Shimada, T
中科院分区：
文献类型：
--
作者：
Ogawa, R;Mizuno, H;Shimada, T
我们之前已经证明，脂肪来源的基质细胞（ASCs）和骨髓来源的基质细胞（BSCs）在体外和体内都能分化成多种细胞系。这两种类型都被认为包括间充质干细胞。利用绿色荧光蛋白（GFP）转基因小鼠的同质标记细胞，我们之前也报道了BSCs和ASCs的可塑性。在这项研究中，我们重点研究了从GFP转基因小鼠中收获的ASCs在体外的成脂分化。同时收获前脂肪细胞和成熟脂肪细胞，培养后与ASCs进行比较。利用转基因GFP小鼠腹股沟脂肪垫分离ASCs、前脂肪细胞和成熟脂肪细胞。扩增至三代ASCs后，细胞在成脂培养基中孵育两周。通过油红O染色和脂肪细胞特异性过氧化物酶体增殖激活受体γ ma2 （ppar - γ ma2）基因的表达评估ASCs的成脂分化。这些ASCs染色呈阳性，并检测到PPAR-gamma2的表达。此外，我们还试图表征性别差异对雄性和雌性小鼠ASCs成脂分化的影响。这是通过实时PCR检测PPAR-gamma2基因的表达水平来评估的。结果表明，雌性小鼠的ASCs表达量最高为雄性小鼠的2.89倍。提示ASCs的成脂分化与性别差异密切相关。(C) 2004爱思唯尔公司版权所有。
We have previously demonstrated that adipose-derived stromal cells (ASCs) as well as bone marrow-derived stromal cells (BSCs) differentiate into a variety of cell lineages both in vitro and in vivo. Both types are considered to include mesenchymal stem cells. Taking advantage of homogeneously marked cells from green fluorescent protein (GFP) transgenic mice, we have also previously reported the plasticity of BSCs and ASCs. In this study, we focused on adipogenic differentiation in vitro by ASCs harvested from GFP transgenic mice. Moreover, preadipocytes and mature adipocytes were harvested at the same time, and the cells were cultured to compare them with ASCs. Inguinal fat pads from GFP transgenic mice were used for the isolation of ASCs, preadipocytes, and mature adipocytes. After expansion to three passages of ASCs, the cells were incubated in an adipogenic medium for two weeks. Adipogenic differentiation of ASCs was assessed by Oil Red O staining and the expression of the adipocyte specific peroxisome proliferative activated receptor gamma2 (PPAR-gamma2) gene. These ASCs stained positively, and expression of PPAR-gamma2 was detected. Moreover, we also tried to characterize the influence of sex differences on the adipogenic differentiation of ASCs harvested from both male and female mice. This was assessed by the expression levels of the PPAR-gamma2 gene using real-time PCR. The results showed that the expression levels of ASCs harvested from female mice were a maximum of 2.89 times greater than those harvested from male mice. This suggests that the adipogenic differentiation of ASCs is closely related to sex differences. (C) 2004 Elsevier Inc. All rights reserved.