Growth, differentiation, and biochemical signatures of rhesus monkey mesenchymal stem cells
Growth, differentiation, and biochemical signatures of rhesus monkey mesenchymal stem cells
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DOI：
10.1089/scd.2007.0076
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发表时间：
2008-02-01
影响因子：
4
通讯作者：
Tarantal, Alice F.
中科院分区：
文献类型：
--
作者：
Kim, Brandon S.;Lee, C. Chang I.;Tarantal, Alice F.
本研究的目的是比较不同年龄组（胎儿、新生儿、婴儿、少年）恒河猴间充质干细胞（rhMSCs）的生长和分化潜力，并利用共聚焦微拉曼光谱评估单个rhMSCs的内在生物分子特征。结果表明，与其他年龄组（新生儿51.9 +/- 2.3 h，婴儿38.2 +/- 3.1 h，幼年40.7 +/- 4.1 h）相比，胎儿细胞在对数生长期的群体倍增率（23.3 +/- 1.3 h）显著低于其他年龄组（66.5 +/- 6.5 h）。与年老动物的细胞相比，胚胎间充质干细胞也以更快的速度向成骨和脂肪谱系分化。拉曼光谱分析显示，与幼年鼠间充质干细胞相比，胎儿的DNA含量更高，蛋白质浓度更低，尽管不同年龄组的光谱具有许多相似的特征。此外，用于区分rhMSCs的主成分分析（PCA）支持先前的发现，即培养的rhMSCs由异质细胞群组成。尽管来自年轻年龄组的rhMSCs的生长潜力得到了证实，但需要进一步的研究来充分探索拉曼显微光谱在表征干细胞和祖细胞（如rhMSCs）方面的潜在用途。
The goal of this study was to compare the growth and differentiation potential of rhesus monkey mesenchymal stem cells (rhMSCs) from different age groups (fetal, newborn, infant, juvenile), and to use confocal micro-Raman spectroscopy to assess the intrinsic biomolecular profiles of individual rhMSCs. Results indicated that fetal cells had significantly shorter population doubling times during the log growth phase (23.3 +/- 1.3 h) and greater population doubling times (66.5 +/- 6.5) when compared to other age groups (newborn 51.9 +/- 2.3, infant 38.2 +/- 3.1, juvenile 40.7 +/- 4.1). Fetal rhMSCs also differentiated toward osteogenic and adipogenic lineages at a faster rate when compared to cells from older animals. The Raman spectral analysis showed greater DNA and lower protein concentration in fetal compared to juvenile rhMSCs, although the spectra from different age groups shared many similar features. Additionally, principal component analysis (PCA), which is used to discriminate between rhMSCs, supported prior findings that suggested that cultured rhMSCs consist of a heterogeneous cell population. Although the growth potential of rhMSCs from the younger age groups was confirmed, further studies will be necessary to fully explore the potential usefulness of Raman micro-spectroscopy to characterize stem and progenitor cells such as rhMSCs.