Metabolism of cinnamic acids by some Clostridiales and emendation of the descriptions of Clostridium aerotolerans, Clostridium celerecrescens and Clostridium xylanolyticum
Metabolism of cinnamic acids by some Clostridiales and emendation of the descriptions of Clostridium aerotolerans, Clostridium celerecrescens and Clostridium xylanolyticum
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DOI：
10.1099/00207713-51-6-2105
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发表时间：
2001-11-01
影响因子：
2.8
通讯作者：
Labat, M
中科院分区：
文献类型：
--
作者：
Chamkha, M;Garcia, JL;Labat, M
研究了耐气性梭状芽孢杆菌DSM5434(T)、新冠冠状芽孢杆菌DSM5628(T)、甲氧基苯并呋喃梭菌DSM 12182(T)、斯特氏梭菌ATCC 35414(T)、亚端梭菌DSM2636、白蚁梭菌DSM5398(T)、耐热乳酸梭菌DSM2910(T)、热释热梭菌DSM5974(T)和木聚糖溶血性梭菌DSM6555(T)对肉桂酸及其衍生物的代谢能力。只有DSM 5434T和C.xylanolyticum DSM 6555T这两个近缘种能将肉桂酸转化为3-苯丙酸。这两个物种还将各种肉桂酸衍生物，包括o-、m-和p-香豆酸、o-、m-和p-甲氧基肉桂酸、p-甲基肉桂酸、咖啡酸、阿魏酸、异阿魏酸和3，4，5-三甲氧基肉桂酸还原为相应的3-苯丙酸衍生物。然而，耐气性弧菌DSM 5434T也能将对香豆酸脱羧基生成4-乙烯基苯酚，然后再还原为4-乙基苯酚。在梭状芽胞杆菌的XIVa亚群中，Celerecresens与气态耐气杆菌和木聚糖菌聚在一起。Celerecrescens DSM 5628T只将间甲氧基肉桂酸和对甲氧基肉桂酸和对甲基肉桂酸代谢成相应的3-苯丙酸衍生物，减少了C-3脂肪族侧椅上的双键。添加葡萄糖显著提高了这三个菌种的生物转化产率。在这些观察的基础上，提出了对耐气性弧菌、新冠弧菌和木解弧菌的描述。
The ability of Clostridium aerotolerans DSM 5434(T), Clostridium celerecrescens DSM 5628(T), Clostridium methoxybenzovorans DSM 12182(T), Clostridium stercorarium ATCC 35414(T), Clostridium subterminale DSM 2636, Clostridium termitidis DSM 5398(T), Clostridium thermolacticum DSM 2910(T), Clostridium thermopalmarium DSM 5974(T) and Clostridium xylanolyticum DSM 6555(T) to metabolize cinnamic acid and various derivatives, with or without glucose supplementation, was examined. Only C. aerotolerans DSM 5434T and C. xylanolyticum DSM 6555T, closely related species, transformed cinnamic acid to 3-phenylpropionic acid. Both species also reduced a wide range of cinnamic acid derivatives, including o-, m- and p-coumaric, o-, m- and p-methoxycinnamic, p-methylcinnamic, caffeic, ferulic, isoferulic and 3,4,5-trimethoxycinnamic acids to their corresponding 3-phenylpropionic acid derivatives. C. aerotolerans DSM 5434T, however, also decarboxylated p-coumaric acid into 4-vinylphenol, which was then reduced to 4-ethylphenol. C. celerecrescens was grouped with C. aerotolerans and C. xylanolyticum in subcluster XIVa of the Clostridiales. C. celerecrescens DSM 5628T only metabolized m- and p-methoxycinnamic and p-methylcinnamic acids to their corresponding 3-phenylpropionic acid derivatives, reducing the double bond in the C-3 aliphatic side chair. Addition of glucose markedly increased the yield of the biotransformations by these three species. An emendation of the descriptions of C. aerotolerans, C. celerecrescens and C. xylanolyticum is proposed, based on these observations.