Inhibition Shapes Sex Selectivity in the Mouse Accessory Olfactory Bulb
Inhibition Shapes Sex Selectivity in the Mouse Accessory Olfactory Bulb
复制标题
DOI：
10.1523/jneurosci.2715-08.2008
复制
发表时间：
2008-11-19
影响因子：
5.3
通讯作者：
Holy, Timothy E.
中科院分区：
文献类型：
--
作者：
Hendrickson, Rebecca C.;Krauthamer, Sandra;Holy, Timothy E.
在整个神经系统中发现了横向连接的抑制回路，包括许多早期的感觉处理系统。然而，在嗅觉等缺乏简单的刺激空间二维表示的系统中，它在塑造神经元刺激选择性方面发挥作用的程度仍然存在争议。我们研究了这个问题，使用的实验准备，允许电生理记录从附件的嗅球的麻醉小鼠在受控传递的信息素刺激，在这种情况下，来自男性和女性小鼠的尿液。我们发现，单个神经元通常对尿液供体的性别具有高度选择性。检查明确的抑制反应，以及男性和女性尿液的混合物的反应，发现横向连接的抑制是普遍的和大幅度的，特别是男性选择性神经元。这种抑制的药理学操作导致许多神经元的刺激选择性发生变化。最后，我们发现，行为反应（怀孕块）引起的不熟悉的男性尿液的存在下，可以抑制女性尿液的刺激，表明该系统显示出与神经抑制的行为一致性。总之，这些结果表明，侧连接的抑制电路在副嗅球中起着重要的作用，在塑造神经的选择性自然刺激。
Laterally connected inhibitory circuitry is found throughout the nervous system, including many early sensory processing systems. The extent to which it plays a role in shaping neuronal stimulus selectivity in systems like olfaction, however, which lack a simple two-dimensional representation of their stimulus space, has remained controversial. We examined this issue using an experimental preparation that allowed electrophysiological recording from the accessory olfactory bulb of an anesthetized mouse during the controlled delivery of pheromonal stimuli, in this case derived from the urine of male and female mice. We found that individual neurons were often highly selective for the sex of the urine donor. Examination of both explicitly inhibitory responses, as well as responses to mixtures of male and female urine, revealed that laterally connected inhibition was both prevalent and of large magnitude, particularly for male-selective neurons. Pharmacological manipulation of this inhibition resulted in a shift in many neurons' stimulus selectivities. Finally, we found that a behavioral response (pregnancy block) evoked by the presence of unfamiliar male urine could be suppressed by the addition of female urine to the stimulus, demonstrating that this system displays a behavioral opponency consistent with neural inhibition. Together, these results indicate that laterally connected inhibitory circuitry in the accessory olfactory bulb plays an important role in shaping neural selectivity for natural stimuli.