Genetic and developmental analysis of polytene section 17 of the X chromosome of Drosophila melanogaster.
Genetic and developmental analysis of polytene section 17 of the X chromosome of Drosophila melanogaster.
复制标题
果蝇 X 染色体第 17 段多线的遗传和发育分析。
作者：
Daniel F. Eberl;L. Perkins;M. Engelstein;A. Hilliker;Norbert Perrimon
黑腹果蝇X染色体的多线区17此前已知含有六个对卵子发生和胚胎发生重要的推定致死互补群，现在已在遗传和发育方面进一步表征。我们构建了fcl+Y，这是该区域的重复，这使我们能够对该区域进行特异性诱变筛选并进行互补分析（以前不可能）。我们发现了67个新的致死突变，它们在Df（1）N19中定义了15个互补组，Df（1）N19删除了大部分多线17节。合子致死表型的这些和预先存在的突变多线第17节内进行了检查，并在纯合子生殖系克隆显性雌性不育技术进行了分析，他们的母亲的要求。我们提出的证据表明，产生两种发育调节转录本的额外基因位于该区域并参与胚胎发生，尽管没有发现该基因的突变。在这37 ~ 43条多线染色体带中，我们确定了17个基因，其中12个（71%）与卵子发生或胚胎发生有关。
Polytene section 17 of the X chromosome of Drosophila melanogaster, previously known to contain six putative lethal complementation groups important in oogenesis and embryogenesis, has here been further characterized genetically and developmentally. We constructed fcl+Y, a duplication of this region, which allowed us to conduct mutagenesis screens specific for the region and to perform complementation analyses (previously not possible). We recovered 67 new lethal mutations which defined 15 complementation groups within Df(1)N19 which deletes most of polytene section 17. The zygotic lethal phenotypes of these and preexisting mutations within polytene section 17 were examined, and their maternal requirements were analysed in homozygous germline clones using the dominant female sterile technique. We present evidence that an additional gene, which produces two developmentally regulated transcripts, is located in this region and is involved in embryogenesis, although no mutations in this gene were identified. In this interval of 37 to 43 polytene chromosome bands we have defined 17 genes, 12 (71%) of which are of significance to oogenesis or embryogenesis.