THE EQUILIBRIUM CONSTANTS AND OXIDATION-REDUCTION POTENTIALS OF SOME THIOL-DISULFIDE SYSTEMS
THE EQUILIBRIUM CONSTANTS AND OXIDATION-REDUCTION POTENTIALS OF SOME THIOL-DISULFIDE SYSTEMS
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DOI：
10.1021/ja01574a005
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发表时间：
1957-01-01
影响因子：
15
通讯作者：
PIHL, A
中科院分区：
文献类型：
--
作者：
ELDJARN, L;PIHL, A
图1. CSH+ RS* S* R系统的三种标记组分的纸电泳分离：A，将2.50 X 10-3 M CSH与0.54 X 10-3 M RS* S* R在pH 7.4和37下孵育15分钟。将混合物酸化至pH 2，并在乙酸汞处理的纸上进行电泳。详情请参阅正文。B和C分别是RS* H和RS* S* R的纸电泳。在每个平衡实验中，分析8至14个单独的样品。各峰内放射性的平均百分比用于计算相应化合物的平衡浓度。用差分法求得其余两种未标记组分的平衡浓度，并计算其平衡常数。虽然
Fig. 1.—The paper electrophoretic separation of the three labeled components of the CSH+ RS* S* Rsystem: A, 2.50 X 10-3 M CSH was incubated for 15 minutes with 0.54 X 10-3 M RS* S* R at pH 7.4 and 37. The mixture was acidified to pH 2 and subjected to electrophoresis in mercuric acetate treated paper. For further details see the text. B and C, paper electrophoresis of RS* H and RS* S* R, respectively.In each equilibrium experiment, 8 to 14 separate samples were analyzed. The average percentage of radioactivity within each peak was used for the calculation of theequilibrium concentration of the corresponding compound. The equilibrium concentrations of the two remaining unlabeled constituents were obtained by difference, and the equilibrium constants were subsequently calculated. Although