TRANSMISSION OF XANTHOMONAS-PHASEOLI IN SEED OF RESISTANT AND SUSCEPTIBLE PHASEOLUS GENOTYPES
TRANSMISSION OF XANTHOMONAS-PHASEOLI IN SEED OF RESISTANT AND SUSCEPTIBLE PHASEOLUS GENOTYPES
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DOI：
10.1094/phyto-70-638
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发表时间：
1980-01-01
期刊：
影响因子：
3.2
通讯作者：
SAETTLER, AW
中科院分区：
文献类型：
--
作者：
CAFATI, CR;SAETTLER, AW
研究了菜豆白粉病病原菌X.phaseoli(=X.campestris)(XP)在大白菜豆(P.acutifolius)种子和‘大北方’(Great Northern)(G.N.)内布拉斯加州1号选择27，品种(G.N.)谷地、栽培品种(G.N.)朱尔斯、育种品系MSU-51319(均为中抗)和品种托斯可拉、海尔(均为感病品种)。用装有R15-1(抗50ppm利福平的XP突变体)悬浮液的注射器在发育平坦的绿色阶段抓取豆荚背缝的方法接种田间植株，每毫升10个集落形成单位。在正常成熟期，只有敏感的‘’托斯可拉‘’有典型的白叶枯病症状。在含有利福平的固体和液体中都分离到了内传的枯萎病菌。突变体R15-1分别从40、42、46、51、42和70%的内布拉斯加州特帕里、内布拉斯加州1号选择27、‘山谷’、‘朱尔斯’、msu-51319和‘托斯可拉’的种子中恢复。突变体R15-1的回收率分别为1.3%、2.0%、1.3%、1.9%、2.0%和10.4%。检测种子传播的细菌疫病的测试应包括在所有干豆品种的经认证的无疫病种子的生产计划中。
A study was conducted on the possible transmission of the bean common blight pathogen X. phaseoli (= X. campestris) (Xp) in seed of resistant P. acutifolius (tepary bean), and in P. vulgaris genotypes ''Great Northern'' (G. N.) Nebraska # 1 selection 27, cultivar (G. N.) Valley, cultivar (G. N.) Jules, and breeding line MSU-51319 (all moderately resistant) and cultivars Tuscola and Seafarer (both susceptible). Field-grown plants were inoculated by scratching the dorsal suture of pods at the flat green stage of development with a syringe containing a suspension of R15-1 (an Xp mutant resistant to 50 ppm rifampin) at 10 colony-forming units per milliliter. At normal maturity, only susceptible ''Tuscola'' had typical bacterial blight symptoms. Internally borne blight bacteria were isolated in both solid and liquid rifampin-containing media. Mutant R15-1 was recovered from 40, 42, 46, 51, 42 and 70% of seeds with any type of visible symptoms of internal blight infection in the genotypes tepary, Nebraska # 1 selection 27, ''Valley'', ''Jules'', MSU-51319, and ''Tuscola'', respectively. Mutant R15-1 also were recovered from 1.3, 2.0, 1.3, 1.9, 2.0, and 10.4% of symptomless seeds of the same genotypes, respectively. Tests to detect seed-borne bacterial blight should be included in production programs for certified, blight-free seed of all dry bean cultivars.