Secretory processes in perfused excretory duct of rat submaxillary gland.
Secretory processes in perfused excretory duct of rat submaxillary gland.
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大鼠颌下腺灌注排泄管的分泌过程。
DOI：
10.1152/ajplegacy.1968.215.3.664
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
L. Schneyer
中科院分区：
文献类型：
--
作者：
L. Schneyer
方法选用6月龄以下成年雄性Wistar和Long-Evans大鼠，体重300-450 g。动物可自由获取标准实验室食物和水，直至实验时。此时，注射戊巴比妥钠（50 μ g/kg，ip）并开始外科手术。对于使用胶体示踪剂的实验，将两个颌下腺和每个颌下腺导管的门端的长度（约1cm）从舌下腺和导管轻轻地分离。用充满肝素化盐水的聚乙烯管对一条股静脉进行插管。对于主排泄管的灌注，外科手术与Young等人描述的相似。（20）、这里采用的手术顺序包括插管，首先，使用细聚乙烯管（Clay-Adams PE-10）从一个颌下导管的口端插入，该管有斜面，便于插入（18）。未使用结扎线固定口腔插管。接下来，暴露同侧的颌下腺并将其与粘附的舌下腺分离。将舌下腺导管与颌下导管分离几毫米并结扎。气管插管。最后，使用放大镜，用虹膜剪打开颌下导管的门端，将细尖（80-p）聚乙烯套管远端插入导管内腔约5 mm，并结扎到位。血管标记。胶体碳被用作
METHODSAdult male Wistar and Long-Evans rats under 6 months of age, usually weighing 300-450 g, were used. The animals had free access to standard laboratory chow and water until the time of the experiment. At that time, sodium pentobarbital was injected (50 “g/kg, ip) and surgical procedure begun.Surgical procedure. For experiments with colloidal tracer, both submaxillary glands and a length (about 1 cm) of the hilar end of each submaxillary duct were gently separated from the sublingual glands and ducts. One femoral vein was cannulated with polyethylene tubing filled with heparinized saline. For perfusion of the main excretory duct, the surgical procedure was similar to that described by Young et al.(20). The surgical sequence employed here involved cannulation, first, of the oral end of one submaxillary duct, using fine polyethylene tubing (Clay-Adams PE-10) beveled for easier insertion (18). No ligature was used to retain the oral cannula. Next, the ipsilateral submaxillary gland was exposed and separated from the adhering sublingual gland. The duct of the sublingual gland was separated from the submaxillary duct for a length of several millimeters and ligated. The trachea was cannulated. Finally, using loupes, the hilar end of the submaxillary duct was opened with iris scissors and a fine-tipped(80-p) polyethylene cannula was inserted distally into the lumen of the duct, for a distance of about 5 mm, and ligated in place. Vascular marking. Colloidal carbon was used as a