The origins of replication of granuloviruses
The origins of replication of granuloviruses
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DOI：
10.1007/s00705-008-0148-0
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发表时间：
2008-08-01
影响因子：
2.7
通讯作者：
Winstanley, Doreen
中科院分区：
文献类型：
--
作者：
Hilton, Sally;Winstanley, Doreen
八个颗粒病毒（GV）的基因组，已被分析的同源区域（小时），可能作为复制起点的存在。在苹果蠹蛾GV（Cydia pomonella GV，CpGV）基因组中，11小时内有13个74-76-bp的回文酶被鉴定，并在感染依赖性DNA复制试验中被发现复制。我们在其中一个小时内报告了一个进一步的回文，发现它可以复制，使总数达到14个回文。我们还报告了不完善的回文，与类似的13-bp的末端序列的CpGV回文，在奥拉纳GV，Cryptophlebia leucotreta GV（CrleGV），Choristoneura occurentalis GV和Phthorimaea operculella GV基因组。在小地老虎GV中未检测到hrs，并且在小菜蛾GV和黑穗螟GV基因组中未检测到除已发表的hrs或回文外的额外hrs或回文。在C.使用CpGV依赖性复制测定法测定Pomonella细胞中的DNA含量。发现两个CrleGV小时以低水平复制。
The genomes of eight granuloviruses (GVs), have been analyzed for the presence of homologous regions (hrs) that may act as origins of replication. Thirteen 74-76-bp palindromes within 11 hrs have previously been identified in the Cydia pomonella GV (CpGV) genome and found to replicate in an infection-dependent DNA replication assay. We report a further palindrome within one of the hrs, which was found to replicate, bringing the total to 14 palindromes. We also report imperfect palindromes, with similar 13-bp end sequences to the CpGV palindromes, within the Adoxophyes orana GV, Cryptophlebia leucotreta GV (CrleGV), Choristoneura occidentalis GV and Phthorimaea operculella GV genomes. No hrs were detected in Agrotis segetum GV, and no additional hrs or palindromes, other than those published, were detected in the Plutella xylostella GV and Xestia c-nigrum GV genomes. Several putative hrs from the GVs were tested for replication in C. pomonella cells using a CpGV-dependent replication assay. Two CrleGV hrs were found to replicate at a low level.