Analysis of contaminants in commercial preparations of the tetrapeptide tuftsin by high-performance liquid chromatography.
Analysis of contaminants in commercial preparations of the tetrapeptide tuftsin by high-performance liquid chromatography.
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采用高效液相色谱法分析四肽促尿素商业制剂中的污染物。
DOI：
10.1016/s0021-9673(00)81826-9
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Nishioka,K
中科院分区：
文献类型：
--
作者：
Amoscato,AA;Babcock,GF;Nishioka,K
180用市售的簇藻刺激的NOTES表现出低于正常的吞噬反应，并且通常对簇藻的吞噬、杀菌和杀肿瘤刺激活性没有反应。类似结构的多肽已经被证明是有效的抑制tuftsin刺激活性的抑制剂，这些和其他污染物确实会导致靶细胞反应的差异，并且在某些情况下，可能需要更高的浓度来引发生物活性。因此，在今后的研究中，必须对其纯度进行验证。因此，我们采用反相高效液相色谱法（HPLC）对一种生物无活性的商业制剂进行了详细的研究。在过去的几年中，反相高效液相色谱与离子配对试剂的结合已被用于多肽和蛋白质的分析，并取得了巨大的成功。它比目前用于簇氟辛合成的纯化方法有几个优点，包括相对较快的分析时间，区分外消旋形式的能力以及相对容易适应制备规模。本报告中的色谱系统证明了一种商业制备的簇毛酮的异质性，纯化产物的生物活性比开始样品的生物活性更高。
180 NOTES stimulated with commercially available tuftsin exhibit a lower than normal phagocytic response and very often are unresponsive to tuftsin’s phagocytic, bactericidal and tumoricidal stimulatory activity. Peptides of similar structure have been documented as potent inhibitors of tuftsin’s stimulatory activities l3 These and other contaminants-could indeed lead to discrepancies in target cell response and may, in certain cases, require higher concentrations to elicit biologica activities. Therefore, it is imperative that the_ purity of commercial preparations of tuftsin be verified before their use in future investigations_ Consequently, we undertook a detailed study of a biologically inactive commercial preparation of tuftsin usin, w reversed-phase high-performance liquid chromatography(HPLC).Over the past several years, reversed-phase HPLC in conjunction with ionpairing reagents, has been employed in the analysis of peptides and proteins with great success’~-‘6. It offers several advantages over the purification procedures currently employed in tuftsin syntheses’7-“, including a relatively rapid analysis time, the ability to distinguish racemized forms and the relative ease of adapting it to a preparative scale. The chromatographic system in this report demonstrates the heterogeneity of a commercial preparation of tuftsin with subsequent enhancement of biological activity of the purified product above that of the starting sample.