A serial MR study of cerebral blood flow changes and lesion development following endothelin-1-induced ischemia in rats
A serial MR study of cerebral blood flow changes and lesion development following endothelin-1-induced ischemia in rats
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DOI：
10.1002/mrm.1263
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发表时间：
2001-10-01
影响因子：
3.3
通讯作者：
Lei, H
中科院分区：
文献类型：
--
作者：
Biernaskie, J;Corbett, D;Lei, H
血管收缩肽内皮素-1 （ET-1）先前已被用于大鼠大脑中动脉（MCA）的短暂闭塞。然而，脑血流（CBF）减少的持续时间和再灌注特征尚不清楚。在本研究中，灌注和t -2加权MRI结合组织学表征ET-1注射后的脑灌注动态和病变发展。22只大鼠在靠近MCA的脑内注射ET-1。脑血流减少到对照水平的30-50%，并且在皮层和纹状体中持续了16个小时的显著减少。48小时t -2加权成像测量的病变大小与7天组织学测量的最终梗死大小相关。CBF的持续减少和缺血性病变的逐渐发展类似于人类中风的演变，这表明该模型可能对评估治疗药物有用，特别是当治疗延迟时。(C) 2001 Wiley-Liss, Inc。
The vasoconstrictive peptide endothelin-1 (ET-1) has been used previously to transiently occlude the middle cerebral artery (MCA) in rats. However, the duration of the resulting reduction in cerebral blood flow (CBF) and the reperfusion characteristics are poorly understood. In this study perfusion and T-2-weighted MRI were used together with histology to characterize the cerebral perfusion dynamics and lesion development following ET-1 injection. Twenty-two rats received an intracerebral injection of ET-1 adjacent to the MCA. CBF was reduced to 30-50% of control levels, and a significant reduction persisted for 16 In in the cortex and 7 h in the striatum. The lesion size measured by T-2-weighted imaging at 48 h correlated with the final infarct size measured by histology at 7 d. The sustained reduction in CBF and the gradual development of the ischemic lesion resemble human stroke evolution, suggesting that this model may be useful for evaluating therapeutic agents, particularly when treatment is delayed. (C) 2001 Wiley-Liss, Inc.