The mitochondrial genome sequence of Mus terricolor: comparison with Mus musculus domesticus and implications for xenomitochondrial mouse modeling.
The mitochondrial genome sequence of Mus terricolor: comparison with Mus musculus domesticus and implications for xenomitochondrial mouse modeling.
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DOI：
10.1016/j.gene.2008.04.001
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发表时间：
2008-07
期刊：
影响因子：
3.5
通讯作者：
W. Pogozelski;L. Fletcher;C. A. Cassar;D. Dunn;I. Trounce;C. Pinkert
中科院分区：
文献类型：
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作者：
W. Pogozelski;L. Fletcher;C. A. Cassar;D. Dunn;I. Trounce;C. Pinkert
从系统发育的角度和理解核-线粒体相互作用的角度来看，了解不同鼠种的线粒体DNA （mtDNA）序列是至关重要的，特别是后者影响我们线粒体疾病的异种杂交模型。为此，报道了鼠种土鼠（Mus terricolor）的线粒体基因组序列，并将其与已发表的普通实验室小鼠家鼠（Mus musculus domesticus）菌株C57BL/6J的序列进行了比较。这些物种之所以令人感兴趣，是因为在m.m.家养核背景下产生了携带m.m. terricolcolour mtDNA的异种线粒体杂交小鼠。尽管总共1763个核苷酸替换具有显著的异质性，但其中大多数是沉默的，导致高度保守的蛋白质序列只有159个氨基酸差异。此外，58%的氨基酸差异代表保守取代。tRNA基因与rRNA基因的同源性均在91%以上。控制区显示出最大的异质性，如预期的那样，总体同源性为85%。在保守序列区I、保守序列区III和l链复制起始区均发现100%同源。7个线粒体基因中，复合物I基因的蛋白质编码基因差异最大，氨基酸同源性为91-97%。复合物III和IV基因的同源性在98-100%之间。从这些数据来看，复杂I差异似乎对驯化m.m.和陆色m.m.杂交的生存能力最为关键。此外，这里报道的序列信息应该有助于确定物种间线粒体转移的关键区域，进一步了解线粒体动力学和线粒体病理，以及研究鼠属起源。
Knowledge of the mitochondrial DNA (mtDNA) sequence of divergent murine species is critical from both a phylogenetic perspective and in understanding nuclear-mitochondrial interactions, particularly as the latter influences our xenocybrid models of mitochondrial disease. To this end, the sequence of the mitochondrial genome of the murine species Mus terricolor (formerly Mus dunni) is reported and compared with the published sequence for the common laboratory mouse Mus musculus domesticus strain C57BL/6J. These species are of interest because xenomitochondrial cybrid mice were created that harbor M. terricolor mtDNA in a M. m. domesticus nuclear background. Although the total of 1763 nucleotide substitutions represents striking heterogeneity, the majority of these are silent, leading to highly conserved protein sequences with only 159 amino acid differences. Moreover, 58% of these amino acid differences represented conservative substitutions. All of the tRNA genes and rRNA genes have homology of 91% or greater. The control region shows the greatest heterogeneity, as expected, with 85% homology overall. Regions of 100% homology were found for Conserved Sequence Block I, Conserved Sequence Block III and the L-strand origin of replication. Complex I genes showed the greatest degree of difference among protein-coding genes with amino acid homology of 91–97% among the seven mitochondrial genes. Complexes III and IV genes show high homology ranging from 98–100%. From these data, complex I differences appear most critical for the viability of M. m. domesticus: M. terricolor cybrids. Moreover, the sequence information reported here should be useful in identifying critical regions for mitochondrial transfer between species, for furthering the understanding of mitochondrial dynamics and pathology in transmitochondrial organisms, and for the study of Mus genus origins.