Isolation and Characterization of aMycobacterium Species Capable of Degrading Three- and Four-Ring Aromatic and Aliphatic Hydrocarbons
Isolation and Characterization of aMycobacterium Species Capable of Degrading Three- and Four-Ring Aromatic and Aliphatic Hydrocarbons
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DOI：
10.1128/aem.65.2.549-552.1999
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发表时间：
1999-02
影响因子：
4.4
通讯作者：
S. Churchill;J. P. Harper;P. Churchill
中科院分区：
文献类型：
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作者：
S. Churchill;J. P. Harper;P. Churchill
摘要从多环芳烃污染的淡水沉积物中分离到一株分枝杆菌CH1，并对其16SrDNA序列进行了鉴定。菌株CH 1能够矿化三环和四环多环芳烃，包括菲，芘和荧蒽。此外，菌株CH 1可以利用菲或芘作为唯一的碳源和能源。在芘矿化过程中观察到至少3天的滞后期。当菌株CH 1在菲或荧蒽存在下生长时，该滞后期减少到小于1天。菌株CH 1还能够使用广泛的烷烃作为唯一的碳源和能源。用nahAcgene探针未检测到DNA杂交，表明参与PAH代谢的酶与特征良好的萘双加氧酶基因无关。在高严谨度条件下，用噬油假单胞菌的alkB基因不能检测到DNA杂交。然而，在低严格性条件下存在轻微但可检测的杂交。这表明参与烷烃氧化的基因之间存在遥远的关系。
ABSTRACT Mycobacterium sp. strain CH1 was isolated from polycyclic aromatic hydrocarbon (PAH)-contaminated freshwater sediments and identified by analysis of 16S rDNA sequences. Strain CH1 was capable of mineralizing three- and four-ring PAHs including phenanthrene, pyrene, and fluoranthene. In addition, strain CH1 could utilize phenanthrene or pyrene as a sole carbon and energy source. A lag phase of at least 3 days was observed during pyrene mineralization. This lag phase decreased to less than 1 day when strain CH1 was grown in the presence of phenanthrene or fluoranthene. Strain CH1 also was capable of using a wide range of alkanes as sole carbon and energy sources. No DNA hybridization was detected with the nahAcgene probe, indicating that enzymes involved in PAH metabolism are not related to the well-characterized naphthalene dioxygenase gene. DNA hybridization was not detected when the alkB gene fromPseudomonas oleovorans was used under high-stringency conditions. However, there was slight but detectable hybridization under low-stringency conditions. This suggests a distant relationship between genes involved in alkane oxidation.