Differences in metabolic parameters and gene expression related to osteochondrosis/osteoarthrosis in pigs fed 25-hydroxyvitamin D3
Differences in metabolic parameters and gene expression related to osteochondrosis/osteoarthrosis in pigs fed 25-hydroxyvitamin D3
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DOI：
10.1051/vetres:2002024
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发表时间：
2002-07-01
影响因子：
4.4
通讯作者：
Whitehead, C
中科院分区：
文献类型：
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作者：
Jefferies, D;Farquharson, C;Whitehead, C
骨软骨病/骨关节病（OC/OA）是猪中发生的各种关节病变的常用术语。已经描述了可能导致这些疾病的病理学，但主要原因仍未知。我们假设，由于OC与软骨发育不良有一些相似之处，这涉及生长板软骨细胞无法完全分化和肥大，因此用25-羟基维生素D-3（25-D）治疗可能会降低猪病变的发生率和/或严重程度，就像在软骨发育不良的鸡中一样。对照组猪随意饲喂市售饲料。在处理组中，该饮食中补充有0.1 mg/kg的25-D。在第7、12、16和21周时，对对照组和给药组各10头猪进行采样。25-D治疗对OC/OA病变的发生率或严重程度没有影响。软骨干重、总胶原蛋白含量和蛋白多糖含量以及总钙、无机磷、维生素C、胰岛素样生长因子-I、甲状旁腺激素和肿瘤坏死因子α的血浆水平均不受治疗影响。此外，这些参数均与OC/OA病变的发生率或严重程度无关。RT-PCR检测的23个基因中有21个基因的mRNA表达水平在OA病变样本的关节软骨中与正常关节软骨相比没有改变。然而，在OA病变和病变附近样本中，II型胶原减少，X型胶原增加。这些结果表明，猪的骨关节炎可能与其他哺乳动物的骨关节炎具有某些共同特征。
Osteochondrosis/osteoarthrosis (OC/OA) are common terms for various joint pathologies that occur in pigs. Pathologies that may contribute to these disorders have been described, but the primary cause(s) remain unknown. We hypothesised that as OC has some similarities to dyschondroplasia, which involves a failure of growth plate chondrocytes to fully differentiate and hypertrophy, treatment with 25-hydroxyvitamin D-3 (25-D) might reduce the incidence and/or severity of lesions in pigs, as it does in chickens with dyschondroplasia. Control pigs were fed a commercial diet ad libitum. In the treated group this diet was supplemented with 25-D at 0.1 mg/kg. Ten pigs from each of the control and treated groups were sampled at 7, 12, 16 and 21 weeks. Treatment with 25-D had no effect on the incidence or severity of OC/OA lesions. Cartilage dry weight, total collagen content and proteoglycan content, and plasma levels of total calcium, inorganic phosphorous, vitamin C, insuline-like growth factor-I, parathyroid hormone and tumour necrosis factor alpha were unaffected by treatment. In addition, none of these parameters were correlated with the incidence or severity of OC/OA lesions. The mRNA expression levels of 21 out of 23 genes assayed by RT-PCR were unaltered in articular cartilage from OA lesion samples as compared to normal articular cartilage. However, collagen type II was reduced and collagen type X increased in OA lesion and near lesion samples. These results suggest that OA in pigs may share some features of osteoarthritis in other mammalian species.