Up-regulation of Notch-1 in psoriasis: an immunohistochemical study
Up-regulation of Notch-1 in psoriasis: an immunohistochemical study
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DOI：
10.1016/j.anndiagpath.2011.09.005
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发表时间：
2012-06-01
影响因子：
2
通讯作者：
Elnaidany, Nada Farag
中科院分区：
文献类型：
--
作者：
Abdou, Asmaa Gaber;Maraee, Alaa Hassan;Elnaidany, Nada Farag
Notch通路在包括表皮及其附属物在内的多种生物体和组织中的分化、增殖和影响细胞命运决定中起关键作用。Notch-1在银屑病中的作用尚未得到广泛评价;因此，本研究旨在评价其在这种常见皮肤病发病机制中的作用。目前的研究使用免疫组化技术，以评估35例慢性斑块状银屑病患者的皮损活检组织中Notch-1的表达，并与正常皮肤活检组织（代表对照组）进行比较。Notch-1在正常皮肤和银屑病患者表皮均有表达，但在银屑病患者表皮中表达强度更高，且以核形式表达更常见，且更广泛。根据银屑病面积和严重程度指数评分评估的银屑病恶化与Notch-1表达的强度（P = 0.005）和核形式（P = 0.0001）显著相关。Notch-1的核形式也与女性性别相关（P = 0.043）。从这项研究来看，Notch-1的上调而不是下调可能在银屑病的发病机制中起作用。核形式是导致症状恶化的原因，并且它是可以通过peptide和紫外线A辐射（PUVA）治疗的效果而消失的形式。(C)2012 Elsevier Inc. All rights reserved.
The Notch pathway plays a key role in differentiation, proliferation, and influencing cell fate decision in multiple organisms and tissues including the epidermis and its appendages. The role of Notch-1 in psoriasis has not been widely evaluated; therefore, the current study aimed to evaluate its role in etiopathogenesis of this common skin disease. The current study used immunohistochemical technique to evaluate Notch-1 expression in 35 lesional biopsies of patients having chronic plaque psoriasis in comparison with normal skin biopsies, representing the control group. Notch-1 was expressed in the epidermis of both normal and psoriatic skins; however, the intensity was in favor of psoriatic lesion, and the nuclear form of Notch-1 was more frequently and diffusely seen in psoriasis. Exacerbation of psoriasis as assessed by the Psoriasis Area and Severity Index score was significantly associated with intense (P = .005) and nuclear form of Notch-1 expression (P = .0001). The nuclear form of Notch-1 was also correlated with female sex (P = .043). From this study, up-regulation and not down-regulation of Notch-1 may have a role in pathogenesis of psoriasis. The nuclear form is responsible for the exacerbation of symptoms, and it is the one that may disappear by the effect of psoralen and ultraviolet A radiation (PUVA) therapy. (C) 2012 Elsevier Inc. All rights reserved.