Developmental and glucocorticoid regulation of surfactant protein mRNAs in preterm lambs.
Developmental and glucocorticoid regulation of surfactant protein mRNAs in preterm lambs.
复制标题
早产羔羊表面活性蛋白 mRNA 的发育和糖皮质激素调节。
DOI：
10.1152/ajplung.1999.277.6.l1142
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Ballard,PL
中科院分区：
文献类型：
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作者：
Tan,RC;Ikegami,M;Jobe,AH;Yao,LY;Possmayer,F;Ballard,PL
糖皮质激素处理可增加早产羔羊肺组织和灌洗液中表面活性蛋白A和SP-B的含量。为了研究这一过程，我们确定了SP mRNA的个体发育和糖皮质激素诱导。在单独的治疗方案中，每种方案都有自己的对照组，在早产前15 h、48 h或每周一次给绵羊注射倍他米松1-4次。使用绵羊SP cDNA，我们发现增加等于或超过三倍的基础水平的所有三个SP mRNA之间的125天和长期。在better 3处理后，SP-B和SP-C mRNA水平在15 h内升高，所有SP mRNA在24 h后升高（≥2倍）; better 3处理后1-3周分娩的胎儿的mRNA水平与对照组无差异。我们的结论是，在体内贝替尼迅速诱导SP mRNA的协调增加，这是完全可逆的7天内，尽管重复剂量的贝替尼。SP-A和SP-B的mRNA和蛋白质含量的类似增加表明，糖皮质激素对这些SP在体内的调节主要是翻译前的。
Glucocorticoid treatment increases content of surfactant protein (SP) A and SP-B in lung tissue and lavage fluid of preterm lambs. To investigate this process, we determined the ontogeny and glucocorticoid induction of SP mRNAs. In separate treatment protocols, each with its own controls, sheep were injected with betamethasone 15 h, 48 h, or weekly for 1–4 doses before preterm delivery. Using ovine SP cDNAs, we found an increase equal to or more than threefold in basal levels of all three SP mRNAs between 125 days and term. After betamethasone treatment, SP-B and SP-C mRNA levels increased by 15 h and all SP mRNAs were elevated after 24 h (≥2-fold); mRNA levels in fetuses delivered 1–3 wk after betamethasone were not different from control. We conclude that in vivo betamethasone rapidly induces a coordinated increase in SP mRNAs, which is fully reversible within 7 days despite repetitive doses of betamethasone. Similar increases in mRNA and protein contents for SP-A and SP-B suggest that glucocorticoid regulation of these SPs in vivo is primarily pretranslational.