Antibody to cloned HSV glycoproteins B and D plus adult human leukocytes protect neonatal mice from lethal HSV infection.
Antibody to cloned HSV glycoproteins B and D plus adult human leukocytes protect neonatal mice from lethal HSV infection.
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克隆 HSV 糖蛋白 B 和 D 的抗体加上成人白细胞可保护新生小鼠免受致命的 HSV 感染。
DOI：
10.1016/0166-3542(88)90046-0
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发表时间：
1988
影响因子：
7.6
通讯作者：
Kohl,S
中科院分区：
文献类型：
--
作者：
Bernstein,DI;Loo,LS;Kohl,S
比较了单纯疱疹病毒(HSV)感染豚鼠产生的抗血清和单纯疱疹病毒(HSV)糖蛋白gB和gD免疫豚鼠后产生的抗血清的体外抗体依赖性细胞毒性(ADCC)活性和体内保护作用。豚鼠抗体在体外可与人单核细胞共同参与ADCC，其抗gBgD血清相当于HSV康复血清。在体内，每种豚鼠血清在与人单核细胞一起给药时都能够保护新生小鼠免受致命的HSV-1感染，但单独给药时不能。抗gBgD血清在体内最有效，在10−4稀释度下对17只新生小鼠有15只(88%)具有保护作用，也是唯一一种在10−6稀释度下具有保护作用的豚鼠血清(5只新生小鼠)。
Antisera produced by HSV infection or following vaccination of guinea pigs with the cloned herpes simplex virus (HSV) glycoproteins gB and gD were compared for in vitro antibody-dependent cellular cytotoxicity (ADCC) activity and for in vivo protection. Antibody from guinea pigs was able to participate in ADCC with human mononuclear cells in vitro, anti-gBgD serum being equivalent to HSV convalescent sera. In vivo, each of the guinea pig sera was able to protect neonatal mice from a fatal HSV-1 infection when given with human mononuclear cells but not when given alone. The anti-gBgD serum was the most effective in vivo, protecting 15 of 17 (88%) neonatal mice when given at a 10−4dilution with human mononuclear cells and was the only guinea pig serum protective at a 10−6dilution (5 of 7 neonatal mice).