PROBLEMS OF CD SPECTROMETERS .5. CAN WE MEASURE CD AND LD SIMULTANEOUSLY - COMMENTS ON DIFFERENTIAL POLARIZATION MICROSCOPY (CD AND LINEAR DICHROISM)
PROBLEMS OF CD SPECTROMETERS .5. CAN WE MEASURE CD AND LD SIMULTANEOUSLY - COMMENTS ON DIFFERENTIAL POLARIZATION MICROSCOPY (CD AND LINEAR DICHROISM)
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DOI：
10.1002/bip.360300318
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发表时间：
1990-01-01
期刊：
影响因子：
2.9
通讯作者：
MAEDA, S
中科院分区：
文献类型：
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作者：
SHINDO, Y;NISHIO, M;MAEDA, S
Livolant、Mickols 和 Maestre 发表的一篇关于圆二色性 (CD) 和线性二色性 (LD) 显微镜的有趣论文 [(1988) Biopolymers 27, 1761-1969] 引起了我们的注意。他们报告说，他们已经成功地从强且不相容的各向异性样本中获得的单粒子中解算出数学上真实的 CD 和 LD。如果这是真的，那么他们的数学反卷积方法将为偏振调制光谱学开辟新的可能性。然而，仔细阅读他们的论文后，我们发现他们的结果中存在以下模糊点：（1）他们的光谱仪的硬件； (2) 灵敏度矩阵； (3)采用两种标准品分离CD和LD的方法； (4)平均CD曲线； (5)波长范围300～330nm的圆形强度差散射(CIDS)和线性强度差散射(LIDS)。利用穆勒矩阵分析，我们结合我们的数据提出对上述主题的评论。总之，我们认为很难接受他们声称拥有一种通用且有用的方法来从宏观各向异性样品中观察到的信号中分离出真正的 CD 和 LD 的说法。
An interesting paper on circular dichroism (CD) and lenear dichroism (LD) microscopy by Livolant, Mickols, and Maestre [(1988) Biopolymers 27, 1761-1969] has attracted our attention. They reported that they have succeeded in deconvoluting mathematically true CD and LD from singles obtained from their samples of strong and incompatible anisotropies. If this is true, then their method of mathematical deconvolution will open new possibilities in polarization-modulation spectroscopy. However, after carefully reading through their paper, we found the following obscure points in their results: (1) the hardware of their spectrometer; (2) the sensitivity matrix; (3) the method for separation of CD and LD by using two standards; (4) average CD curves; and (5) circular intensity differential scattering (CIDS) and linear intensity differential scattering (LIDS) in the wavelength range from 300 to 330 nmm. Making use of the Mueller matrix analysis, we present our comments on the above subjects together with our data. In conclusion, we maintain that is is difficult to accept their claim of having a general and useful approach to separating true CD and LD from signals observed in macrospically anisotropic samples.