The effects of graphene nanostructures on mesenchymal stem cells.
The effects of graphene nanostructures on mesenchymal stem cells.
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DOI：
10.1016/j.biomaterials.2014.02.054
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发表时间：
2014-06
期刊：
影响因子：
14
通讯作者：
Sitharaman, Balaji
中科院分区：
文献类型：
--
作者：
Talukdar, Yahfi;Rashkow, Jason T.;Lalwani, Gaurav;Kanakia, Shruti;Sitharaman, Balaji
我们报告了二维石墨烯纳米结构;石墨烯纳米洋葱（GNOs），氧化石墨烯纳米带（GONRs）和氧化石墨烯纳米片（GONP）对人间充质干细胞（MSCs）活力和分化的影响。通过AlamarBlue和钙黄绿素AM活力测定，在5-300 μg/ml的浓度范围内持续24或72小时，评估分散在二硬脂酰-sn-甘油基-3-磷酸乙醇胺-N-[氨基（聚乙二醇）]（DSPE-PEG）中的GNOME、GONR和GONP对脂肪来源的间充质干细胞（adMSC）和骨髓来源的间充质干细胞（bmMSC）的细胞毒性。发现2D石墨烯纳米结构的细胞毒性是剂量依赖性的，而不是时间依赖性的，与未处理的对照相比，小于50 μg/ml的浓度没有显示出显著差异。adMSCs向脂肪细胞和成骨细胞的分化潜能--通过油红O染色和洗脱、碱性磷酸酶活性、钙基质沉积和茜素红S染色表征--在用低浓度（10 μg/ml）和高浓度（50 μg/ml）的三种石墨烯纳米颗粒处理24小时时没有显著改变。透射电子显微镜（TEM）和共聚焦拉曼光谱表明，细胞摄取的只有GnO和GONP。这些结果为使用这些纳米颗粒在潜在的安全剂量作为基于MSC的成像和治疗的离体标记奠定了基础。
We report the effects of two-dimensional graphene nanostructures; graphene nano-onions (GNOs), graphene oxide nanoribbons (GONRs), and graphene oxide nanoplatelets (GONPs) on viability, and differentiation of human mesenchymal stem cells (MSCs). Cytotoxicity of GNOs, GONRs, and GONPs dispersed in distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)] (DSPE-PEG), on adipose derived mesenchymal stem cells (adMSCs), and bone marrow-derived mesenchymal stem cells (bmMSCs) was assessed by AlamarBlue and Calcein AM viability assays at concentrations ranging from 5–300 μg/ml for 24 or 72 hours. Cytotoxicity of the 2D graphene nanostructures was found to be dose dependent, not time dependent, with concentrations less than 50 μg/ml showing no significant differences compared to untreated controls. Differentiation potential of adMSCs to adipocytes and osteoblasts, --characterized by Oil Red O staining and elution, alkaline phosphatase activity, calcium matrix deposition and Alizarin Red S staining-- did not change significantly when treated with the three graphene nanoparticles at a low (10 μg/ml) and high (50 μg/ml) concentration for 24 hours. Transmission electron microscopy (TEM) and confocal Raman spectroscopy indicated cellular uptake of only GNOs and GONPs. The results lay the foundation for the use of these nanoparticles at potentially safe doses as ex vivo labels for MSC-based imaging and therapy.
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