Transcriptome profiling reveals the crucial biological pathways involved in cold response in Moso bamboo (Phyllostachys edulis)
Transcriptome profiling reveals the crucial biological pathways involved in cold response in Moso bamboo (Phyllostachys edulis)
复制标题
转录组分析揭示了毛竹（Phyllostachys edulis）冷反应中涉及的关键生物学途径
DOI：
10.1093/treephys/tpz133
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发表时间：
2020-04-01
期刊：
影响因子：
4
通讯作者：
Zhu, Qiang
中科院分区：
文献类型：
--
作者：
Liu, Yuanyuan;Wu, Chu;Zhu, Qiang
包括毛竹在内的大多数竹种都是热带或亚热带植物，对人类的福祉做出了巨大贡献。低温是制约竹子生长和地理分布的主要环境因子之一。我们对竹子适应冷胁迫过程中分子变化的认识仍然有限。本文通过系统分析低温胁迫下毛竹的转录组学反应，对低温胁迫下毛竹的转录谱进行了综述。结果表明，低温诱导毛竹发生了强烈的形态和生化变化。为了检查响应于冷的整体基因表达变化，使用Illumina测序平台对12个文库（未处理的、在-2 ℃下冷处理0.5、1和24小时）进行测序。本研究在竹子低温响应的早期阶段仅鉴定出少数差异表达基因，而在后期阶段鉴定出大量差异表达基因，表明竹子低温响应基因中大部分为迟发响应基因。来自24个不同家族的共222个转录因子在24 h冷处理过程中差异表达，其中几个著名的C-repeat/dehydration responsive element-binding factor负调控因子的表达在冷处理过程中显著上调，表明存在特殊的冷反应网络。研究结果还表明，与细胞壁和脂肪酸合成相关的基因在低温胁迫下的表达发生了变化，表明它们在竹子抗寒性的获得中可能发挥着重要作用。综上所述，我们的研究表明，毛竹既存在植物界保守的冷胁迫反应途径，又存在种特异性的冷胁迫反应途径，这为进一步研究毛竹冷胁迫反应的调控机制奠定了基础，也为今后通过基因工程手段构建耐冷竹种提供了有用的基因资源。
Most bamboo species including Moso bamboo (Phyllostachys edulis) are tropical or subtropical plants that greatly contribute to human well-being. Low temperature is one of the main environmental factors restricting bamboo growth and geographic distribution. Our knowledge of the molecular changes during bamboo adaption to cold stress remains limited. Here, we provided a general overview of the cold-responsive transcriptional profiles in Moso bamboo by systematically analyzing its transcriptomic response under cold stress. Our results showed that low temperature induced strong morphological and biochemical alternations in Moso bamboo. To examine the global gene expression changes in response to cold, 12 libraries (non-treated, cold-treated 0.5, 1 and 24 h at -2 degrees C) were sequenced using an Illumina sequencing platform. Only a few differentially expressed genes (DEGs) were identified at early stage, while a large number of DEGs were identified at late stage in this study, suggesting that the majority of cold response genes in bamboo are late-responsive genes. A total of 222 transcription factors from 24 different families were differentially expressed during 24-h cold treatment, and the expressions of several well-known C-repeat/dehydration responsive element-binding factor negative regulators were significantly upregulated in response to cold, indicating the existence of special cold response networks. Our data also revealed that the expression of genes related to cell wall and the biosynthesis of fatty acids were altered in response to cold stress, indicating their potential roles in the acquisition of bamboo cold tolerance. In summary, our studies showed that both plant kingdom-conserved and species-specific cold response pathways exist in Moso bamboo, which lays the foundation for studying the regulatory mechanisms underlying bamboo cold stress response and provides useful gene resources for the construction of cold-tolerant bamboo through genetic engineering in the future.