Phosphoproteins altered by antiproliferative doses of human interferon- beta in a human bladder carcinoma cell line.
Phosphoproteins altered by antiproliferative doses of human interferon- beta in a human bladder carcinoma cell line.
复制标题
在人膀胱癌细胞系中，磷蛋白被抗增殖剂量的人干扰素β改变。
DOI：
10.1016/0006-291x(84)90864-7
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发表时间：
1984
影响因子：
3.1
通讯作者：
Hubbell,HR
中科院分区：
文献类型：
--
作者：
Soslau,G;Bogucki,AR;Gillespie,D;Hubbell,HR
用[32P]-ATP标记对照和IFN-β处理的人膀胱癌细胞（RT4）的磷酸化蛋白，并采用聚丙烯酰胺凝胶电泳分析。用抗增殖剂量IFN处理的细胞磷酸化60kd和40kd蛋白水平降低。IFN还在24小时内诱导了22 - 24000分子量范围内的低分子量磷酸蛋白双偶体的修饰。通过体外程序磷酸化高分子量蛋白的能力通常被IFN处理抑制。在ifn处理的细胞中，与43-50,000分子量范围内的蛋白质相关的碱稳定磷酸氨基酸的磷酸化发生了戏剧性的变化。初步研究表明，至少部分ifn诱导的细胞磷酸化蛋白修饰可能是由特定癌基因的转录控制引起的。
Phosphoproteins of control and IFN-β treated human bladder carcinoma cells (RT4) were labelledin vitrowith [32P]-ATP and analyzed by polyacrylamide gel electrophoresis. Cells treated with antiproliferative doses of IFN had reduced levels of phosphorylated 60 Kd and 40 Kd proteins. IFN also induced within 24 hours the modification of a low molecular weight phosphoprotein doublet in the 22–24,000 molecular weight range. The ability to phosphorylate high molecular weight proteins by thein vitroprocedures was generally depressed by IFN treatment. There was a dramatic shift in the phosphorylation of alkali stable phosphoamino acids associated with proteins in the 43–50,000 molecular weight range in IFN-treated cells. Preliminary studies indicate that at least some of the IFN-induced modifications of cellular phosphoproteins may result from transcriptional control of specific oncogenes.